穿心莲内酯对人骨肉瘤细胞的作用及机制  

EFFECT OF ANDROGRAPHOLIDE ON HUMAN OSTEOSARCOMA CELLS AND ITS MECHANISM OF ACTION

作　　者：张希劲 刘小强 高钿[2] 沈若武[1] ZHANG Xijin;LIU Xiaoqiang;GAO Tian;SHEN Ruowu(Department of Special Medicine, School of Basic Medicine, Qingdao University, Qingdao 266021, China)

机构地区：[1]青岛大学基础医学院特种医学系,山东青岛266021 [2]青岛市市立医院产三科

出　　处：《青岛大学学报（医学版）》2020年第4期451-454,共4页Journal of Qingdao University(Medical Sciences)

基　　金：山东省高等学校科技计划项目(L16LK04)。

摘　　要：目的探究穿心莲内酯(AG)对人骨肉瘤(HOS)细胞的作用及机制。方法以正常培养的HOS细胞为对照组,不同浓度AG作用后的HOS细胞为实验组。利用平板克隆实验观察AG对HOS细胞增殖能力的影响,Transwell实验观察AG对HOS细胞侵袭和迁移能力的影响,流式细胞术观察AG对HOS细胞周期分布的影响。结果与对照组相比,实验组HOS细胞的生存率降低,抑制率升高,且呈现出浓度依赖性和时间依赖性,差异均有显著性(F=111.64、35.22,P<0.05)。实验组HOS细胞的增殖能力较对照组降低,也呈现出浓度依赖性,差异有显著性(F=250.95,P<0.05)。与对照组相比,实验组HOS细胞侵袭和迁移能力均降低,差异有显著性(t=21.23、23.36,P<0.05)。与对照组相比,实验组HOS细胞G1期比例降低,S期比例升高,G2期比例降低,差异均有统计学意义(t=4.12~38.88,P<0.05);G1期浓度依赖性不明显(t=0.16~0.40,P>0.05),S期、G2期则表现出一定的浓度依赖性(t=5.41~11.90,P<0.05)。结论AG能够明显降低HOS细胞的生存率,这可能与其影响HOS细胞的增殖能力、侵袭和迁移能力及细胞周期有关。Objective To investigate the effect of andrographolide(AG)on human osteosarcoma(HOS)cells and its mechanism of action.Methods Normally cultured HOS cells were selected as control group and HOS cells treated with different concentrations of AG were selected as experimental group.Plate colony formation assay was used to observe the effect of AG on the proliferative capacity of HOS cells,Transwell assay was used to observe the effect of AG on the invasion and migration abilities of HOS cells,and flow cytometry was used to observe the effect of AG on HOS cell cycle distribution.Results Compared with the control group,the experimental group had a significant reduction in the viability of HOS cells and a significant increase in inhibition rate in a concentration-and time-dependent manner(F=111.64,35.22;P<0.05).Compared with the control group,the experimental group had a significant reduction in proliferative capacity in a concentration-dependent manner(F=250.95,P<0.05),as well as significant reductions in the invasion and migration abilities of HOS cells(t=21.23,23.36;P<0.05).Compared with the control group,the experimental group had a significant reduction in the proportion of cells in G1 phase,a significant increase in the proportion of cells in S phase,and a significant reduction in the proportion of cells in G2 phase(t=4.12-38.88,P<0.05);the change in the cells in G1 phase was not concentration-dependent(t=0.16-0.40,P>0.05),and the changes in the cells in S phase and G2 phase showed a certain degree of concentration dependence(t=5.41-11.90,P<0.05).Conclusion AG can significantly reduce the viability of HOS cells,possibly by affecting the proliferation,invasion,migration,and cell cycle of HOS cells.

关 键 词：穿心莲内酯 骨肉瘤 细胞增殖 细胞运动 细胞周期 

分 类 号：R738.1[医药卫生—肿瘤]