谷氨酸棒杆菌对砷的耐受性及去除效果  

Resistance and Removal Effect by Corynebacterium glutamicum to Arsenic

作　　者：杨新瑶[1] 闫馨予 唐章 陈芳敏 YANG Xinyao;YAN Xinyu;TANG Zhang;CHEN Fangmin(Key Lab of Eco-Restoration of Regional Contaminated Environment,Shenyang University,Shenyang 110044,China;Liaoning Key Lab of Urban Integrated Pest Management and Ecological Security,Shenyang University,Shenyang 110044,China)

机构地区：[1]沈阳大学区域污染环境生态修复教育部重点实验室,辽宁沈阳110044 [2]沈阳大学城市有害生物治理辽宁省重点实验室,辽宁沈阳110044

出　　处：《沈阳大学学报（自然科学版）》2020年第3期200-205,共6页Journal of Shenyang University：Natural Science

基　　金：国家自然科学基金面上项目(41672248);辽宁省特聘教授项目(辽教函[2018]35号);沈阳市中青年科技创新人才团队项目(RC170244).

摘　　要：研究了谷氨酸棒杆菌(Corynebacterium glutamicum ATCC 13032)对不同质量浓度砷(As(V))的耐受性及对不同价态砷(As(Ⅴ)和As(Ⅲ))的去除效果.培养基中的谷氨酸棒杆菌在不同质量浓度砷(As(V))溶液中的生长试验结果显示,此细菌对砷有很强的耐受性,As(V)在低质量浓度(0.5、1.0 mg·L^-1)时促进细菌生长,高质量浓度(10 mg·L^-1)时在反应前期(7 h)明显抑制细菌生长,随反应时间延长(24 h),As(V)对细菌生长有一定促进作用.通过在贫营养的含砷水环境中,对比相同细菌浓度下谷氨酸棒杆菌对As(Ⅴ)和As(Ⅲ)去除效果,结果显示谷氨酸棒杆菌能够通过摄入及吸附2种机理来实现对砷的去除,对As(Ⅴ)的摄入高于As(Ⅲ),但总去除率接近.对比不同细菌浓度下谷氨酸棒杆菌对As(Ⅲ)的去除效果,结果显示,细菌在低细菌浓度条件下对As(Ⅲ)的摄入明显高于高细菌浓度条件,而对As(Ⅲ)的吸附则低于高细菌浓度条件.总体来说,谷氨酸棒杆菌在低细菌浓度下对As(Ⅲ)的去除率高于高细菌浓度条件.The resistance of Corynebacterium glutamicum to different mass concentrations of arsenic(As(Ⅴ))and the removal effect to different valence arsenic(As(Ⅴ)and As(Ⅲ))were studied.The growth experiment results of Corynebacterium glutamicum in different mass concentrations of arsenic(As(Ⅴ))in the culture medium show that this bacterium is very resistant to arsenic.As(Ⅴ)promotes bacterial growth at low mass concentration(0.5,1.0 mg·L^-1),and significantly inhibits bacterial growth at the early stage of reaction(7 h)at high mass concentration(10 mg·L^-1).With the extension of the reaction time(24 h),As(Ⅴ)can promote the growth of bacteria.Comparing the removal effect of Corynebacterium glutamicum to As(Ⅴ)and As(Ⅲ)in different mass concentrations/growth strains in poornutrition arsenic-containing water environments,the results showed that bacteria could achieve the removal of arsenic by ingesting and ads-orption mechanisms,and that the intake of As(Ⅴ)is higher than As(Ⅲ),but the total removal rate is close.The intake of As(Ⅲ)by bacteria at low mass concentration is significantly higher than that by high concentration,while the adsorption of As(Ⅲ)is lower than that by high concentration.The removal rate of As(Ⅲ)by bacteria at low mass concentration is higher than that of high concentration bacteria.

关 键 词：谷氨酸棒杆菌 砷浓度效应 细菌生长状态 耐受性 砷去除 

分 类 号：X172[环境科学与工程—环境科学]