造模后不同时间高压氧治疗对局灶性脑缺血大鼠内源性神经干细胞分化的影响  

Effects of hyperbaric oxygen therapy on differentiation of endogenous neural stem cells in rats with focal cerebral ischemia at different stages

作　　者：麦用军[1] 李翔[1] 郑良佐[1] 阳洪[1] 韦晓谋[1] 薛连璧[2] Mai Yongjun;Li Xiang;Zheng Liangzuo;Yang Hong;Wei Xiaomou;Xue Lianbi(The Fourth Affiliated Hospital of Guangxi Medical University,Liuzhou 545005,Guangxi,China)

机构地区：[1]广西医科大学第四附属医院,广西壮族自治区柳州545005 [2]首都医科大学北京天坛医院

出　　处：《中华航海医学与高气压医学杂志》2020年第2期224-229,共6页Chinese Journal of Nautical Medicine and Hyperbaric Medicine

基　　金：柳州市科委自然科学基金资助项目(2015J030509)。

摘　　要：目的观察造模后不同时间不同疗程高压氧(HBO)治疗对局灶性脑缺血大鼠缺血侧大脑内源性神经干细胞(NSCs)分化的情况。方法采用线栓法复制大脑中动脉阻塞(MCAO)局灶性脑缺血再灌注模型。将105只大鼠按抽签法随机分为假手术组(SS),缺血再灌注模型组(IR),造模后1 d HBO治疗组(IR1d+HBO),造模后7 d HBO治疗组(IR7d+HBO),造模后14 d HBO治疗组(IR14d+HBO),造模后28 d HBO治疗组(IR28d+HBO)。各组造模后第2、4、6、8周分别采用免疫荧光BrdU/β微管蛋白(β-tubulin)、BrdU/胶质纤维酸性蛋白(GFAP)双标法检测缺血侧大脑区新生的神经元及星形胶质细胞双标阳性细胞。结果(1)BrdU/β-tubulin表达量在造模后2周时,SS组11.40±1.52,IR组36.60±2.51,IR1d+HBO组48.60±3.21,IR7d+HBO组42.60±2.30;4周时SS组12.20±1.92,IR组26.20±2.28,IR1d+HBO组39.80±2.17,IR7d+HBO组33.20±1.92,IR14d+HBO组29.20±1.48;6周时SS组12.40±1.67,IR组18.60±1.82,IR1d+HBO组30.20±2.49,IR7d+HBO组25.40±2.79,IR14d+HBO组21.40±2.50,IR28d+HBO组19.60±1.67;8周时SS组11.80±1.64,IR组16.40±2.07,IR1d+HBO组24.40±1.95,IR7d+HBO组21.20±1.48,IR14d+HBO组18.40±1.67,IR28d+HBO组15.60±1.82。各组间BrdU/β-tubulin表达量差异均有统计学意义(P<0.01);与SS组比较,各时间点IR组大鼠BrdU/β-tubulin表达量均增高(P<0.05);各时间点IR1d+HBO、IR7d+HBO组大鼠BrdU/β-tubulin表达量均高于IR组,而各时间点IR14d+HBO、IR28d+HBO组与IR组比较,差异无统计学意义(P>0.05);除SS组,其余各组均在2周时BrdU/β-tubulin表达量达到最高(P<0.05)。(2)BrdU/GFAP表达量在造模后2周时,SS组22.60±1.82,IR组59.00±3.67,IR1d+HBO组50.60±2.51,IR7d+HBO组55.20±2.58;4周时SS组22.20±1.48,IR组45.00±2.24,IR1d+HBO组35.80±1.64,IR7d+HBO组40.20±2.16,IR14d+HBO组44.60±2.51;6周时SS组22.80±1.64,IR组26.68±1.78,IR1d+HBO组27.40±1.67,IR7d+HBO组28.40±1.51,IR14d+HBO组26.20±1.78,IR28d+HBO组26.20±1.48;8周时SS组21.60±1.81,IR组21.40±1.14,IR1d+HBO组24.00±1.58,IR7d+Objective To observe the differentiation of endogenous neural stem cells(NSCs)in the ischemic brain of rats with focal cerebral ischemia at different stages with various treatments.Methods The model of focal cerebral ischemia was established by middle cerebral artery occlusion(MCAO).A total of 105 rats were randomly divided into sham-surgery group(SS),ischemia-reperfusion group(IR),receiving hyperbaric oxygen(HBO)1 day after modeling group(IR1d+HBO),receiving HBO 7 days after modeling group(IR7d+HBO group),receiving HBO 14 days after modeling group(IR14d+HBO group),and receiving HBO 28 days after modeling group(IR28d+HBO group).At the 2nd,4th,6th and 8th week after modeling,the cells of newly generated neurons and astrocyte in the ischemic area were positive in both immunofluorescence BrdU/β-tubulin and BrdU/glial fibrillary acidic protein(GFAP)test were detected.Results Two weeks after modeling,the expressions of BrdU/β-tubulin were 11.40±1.52 in the SS group,36.60±2.51 in the IR group,48.60±3.21 in the IR1d+HBO group,and 42.60±2.30 in the IR7d+HBO group;four weeks after modeling,it was 12.20±1.92 in the SS group,26.20±2.28 in the IR group,39.80±2.17 in the IR1d+HBO group,33.20±1.92 in the IR7d+HBO group,29.20±1.48 in the IR14d+HBO group.Six weeks after modeling,it was 12.40±1.67 in the SS group,18.60±1.82 in the IR group,30.20±2.49 in the IR1d+HBO Group,25.40±2.79 in the IR7d+HBO group,21.40±2.50 in the IR14d+HBO group,19.60±1.67 in the IR28d+HBO group.Eight weeks after modeling,it was 11.80±1.64 in the SS group,16.40±2.07 in the IR group,24.40±1.95 in the IR1d+HBO group,21.20±1.48 in the IR7d+HBO group,18.40±1.67 in the IR14d+HBO group,and 15.60±1.82 in the IR28d+HBO group.The differences in the expressions of BrdU/β-tubulin between these groups were statistically significant(P<0.01).Compared with the SS group,the expression of BrdU/β-tubulin increased in the IR group at each time point(P<0.05).The expressions of BrdU/β-tubulin in the IR1d+HBO and the IR7d+HBO were higher than those i

关 键 词：高压氧 脑缺血 神经干细胞 大鼠 动物模型 

分 类 号：R-332[医药卫生] R74