辐射敏感基因Pig-a、Gadd45α和Hprt作为潜在生物剂量计的实验研究  

Investigating the use of radiation-sensitive genes Pig-a,Gadd45α,and Hprt as potential biodosimeters

作　　者：杨学琴 毛鑫玉 陈钰婷 李悦兰 张文 YANG Xueqin;MAO Xinyu;CHEN Yuting;LI Yuelan;ZHANG Wen(Department of Pathology and Toxicology,Shenzhen Prevention and Treatment Center for Occupational Diseases,Shenzhen 518020,China;Department of Health Inspection and Quarantine,College of Medical Laboratory,Hebei North University,Zhangjiakou 075000,China)

机构地区：[1]深圳市职业病防治院病理毒理所,深圳518020 [2]河北北方学院医学检验学院,张家口075000

出　　处：《辐射研究与辐射工艺学报》2020年第3期46-53,共8页Journal of Radiation Research and Radiation Processing

基　　金：广东省医学科学技术研究基金(A2020147)资助。

摘　　要：为了提高对辐射遗传损伤效应的检测能力,同时建立一种快速简便的生物剂量估算方法,选择Piga、Gadd45α及Hprt 3个基因,运用其mRNA表达水平的变化,筛选可能作为电离辐射生物剂量计的辐射敏感基因。采用RT-qPCR检验非放射从业健康人群(对照组)和放射从业人员(暴露组)外周血中目的基因的相对表达量,分析辐射敏感基因本底表达量的平均值、波动范围和变异系数;应用不同剂量(0 Gy、0.10 Gy、0.25 Gy、0.50 Gy、1.00 Gy、2.00 Gy、5.00 Gy)的X射线照射健康成年人外周血,6 h后用RTqPCR检验目的基因的相对表达量,二项式曲线回归分析存在的剂量–效应关系。结果表明:Pig-a、Gadd45α基因在对照组中本底水平差异较小;Hprt基因本底水平差异较大,变异系数大于20%;Hprt基因在对照组中表达量高于暴露组;Gadd45α基因与Pig-a基因在对照组中的表达量低于暴露组,两组比较有统计学意义(p<0.05)。Gadd45α、Pig-a基因表达量的本底水平与照射剂量存在剂量–效应关系,提示Pig-a基因与Gadd45α基因表达量的变化可能作为电离辐射生物剂量计应用。This study aimed to improve the ability to detect the effects of radiation-associated genetic damage and establish a rapid and simple method for estimating biological dose.Radiation-sensitive genes were selected as biodosimeters from Pig-a,Gadd45α,and Hprt,based on their mRNA expression level changes.RT-qPCR was used to assay the relative expression level of the target genes in peripheral blood samples of healthy non-radiation(controlgroup)and radiation workers(exposed group)and their relative expression level average,fluctuation range and variation coefficient of the background expression were counted.Peripheral blood samples of healthy adults were irradiated with different X-ray doses of 0 Gy,0.10 Gy,0.25 Gy,0.50 Gy,1.00 Gy,2.00 Gy,and 5.00 Gy,respectively.6 h later,the relative expression of the target genes was examined using RT-qPCR and the binomial regression curve analysis was performed to analyze the quantitative effect relationship.The results showed that Piga and Gadd45αexpressions had small background level differences in the control group;Hprt had large background level differences and its variation coefficients were greater than 20%.The Hprt expression level in the control group was higher than that in the exposed group;Gadd45αand Pig-a expressions in the control group were lower than that in the exposed group(p<0.05).There was a dose-effect relationship between the Gadd45αand Pig-a expression levels and irradiation doses,indicating that the Pig-a and Gadd45αexpression level changes could be used as potential biodosimeters.

关 键 词：辐射敏感基因 生物剂量计 Gadd45α 基因 HPRT 基因 Pig-a基因 

分 类 号：TL818[核科学技术—核技术及应用]