精制鱼油胆固醇测定在国标GB 5009.128-2016的应用与优化  

Application and optimization of cholesterol determination of refined fish oil in GB 5009.128-2016

作　　者：温恺嘉 林元亨 李咏华 梁北梅 康丹瑜 王国财 唐顺之 陈文 WEN Kai-Jia;LIN Yuan-Heng;LI Yong-Hua;LIANG Bei-Mei;KANG Dan-Yu;WANG Guo-Cai;TANG Shun-Zhi;CHEN Wen(Guangzhou Baiyun Mountain Hanfang Modern Pharmaceutical Co.,Ltd.,National Engineering Research Center for Modernization of Extraction and Separation Process of TCM,Guangdong Provincial Key Laboratory of pharmaceutical lipids,Guangzhou 510240,China)

机构地区：[1]广州白云山汉方现代药业有限公司,中药提取分离过程现代化国家工程研究中心,广东省药用脂质重点实验室,广州510240

出　　处：《食品安全质量检测学报》2020年第5期1585-1590,共6页Journal of Food Safety and Quality

基　　金：广州市科技计划项目产学研协同创新专题项目(201802030002)。

摘　　要：目的依据GB 5009.128-2016《食品安全国家标准食品中胆固醇的测定》,建立适用于精制鱼油胆固醇测定的分析方法。方法以国标所述为依据,对样品皂化加热方式及冷却放置时间进行了优化,对比了不同测定方法的专属性,最终利用气相色谱法进行测定。结果该方法专属性好,胆固醇含量在2.4993~199.9404μg/mL范围内线性关系好,线性方程为Y=0.2649X-0.2498,r^2为1.0000,定量限达到0.8 ppm,含量精密度的相对标准偏差为2.7%(n=12),回收率在101.5%~106.5%之间。结论该方法快捷、高效、专属性强,适用于精制鱼油的胆固醇含量测定。Objective To establish an analytical method for the determination of cholesterol in refined fish oil according to GB 5009.128-2016 National food safety standard-Determination of cholesterol in food.Methods On the basis of GB 5009.128-2016,the saponification heating method and the cooling time of samples were optimized,and the specificity of different methods was compared.Finally,the determination was carried out by gas chromatography.Results This method had good specificity.The liner relationship was good in the cholesterol content range of 2.4993-199.9404μg/mL.The linear equation was Y=0.2649 X-0.2498 and r^2 was 1.0000.The limit of quantification was 0.8 ppm,the relative standard deviation of content precision was 2.7%(n=12),and the recovery rate was between 101.5%-106.5%.Conclusion This method is rapid,efficient and specific,which is suitable for the determination of cholesterol in refined fish oil.

关 键 词：精制鱼油 胆固醇 气相色谱 

分 类 号：TS225.24[轻工技术与工程—粮食、油脂及植物蛋白工程]