彩色马蹄莲转录组测序及其特性分析  

Transcriptome sequencing and characteristic analysis of Zantedeschia hybrida

作　　者：周琳[1] 张永春[1] 蔡友铭[1] 杨柳燕[1] ZHOU Lin;ZHANG Yongchun;CAI Youming;YANG Liuyan(Forest&Fruii Tree Research Institute,Shanghai Academy of Agricultural Sciences,Shanghai Key Laboratory of Protected Horticultural Technology,Shanghai 201403,China)

机构地区：[1]上海市农业科学院林木果树研究所,上海市设施园艺技术重点实验室,上海201403

出　　处：《上海农业学报》2020年第3期1-8,共8页Acta Agriculturae Shanghai

基　　金：上海市现代农业产业技术体系建设[沪农科产字(2019)第8号];上海市农业科技成果转化项目[沪农科转字(2016)第2-2号]。

摘　　要：为开展彩色马蹄莲基因功能分析、表型差异研究、分子标记开发和遗传多样性研究,通过Illumina HiSeq 4000高通量测序平台对2个彩色马蹄莲育成品种‘金丝绒’和‘梦幻’进行转录组测序分析,经de novo组装后获得76 060条unigene,进一步利用4个公共数据库(Nr、Swiss-Prot、KOG和KEGG)对其进行注释,注释了30 321条unigene;并基于转录组数据开展SSR位点预测和密码子使用偏好性分析。结果表明:有10 083个unigene参与了132条KEGG代谢通路,其中代谢途径和次生代谢产物的生物合成途径是unigene最为富集的2个途径;9 721条unigene序列中共含有13 206个SSR位点;预测到1 115个转录因子,分属于54个家族。此外,彩色马蹄莲密码子使用偏好性较弱,高频密码子为AGG、CAG和AAG。In order to carry out gene function analysis,phenotypic difference research,molecular marker development and genetic diversity research,transcriptome sequencing analysis of two colored calla cultivars‘Jinsirong’and‘Menghuan’were performed by using Illumina hiseq 4000 high-throughput sequencing platform.76060 unigenes were obtained after de novo assembly,which were further annotated with 4 public databases(NR,Swiss prot,KOG and KEGG),and 30321 unigenes were annotated.Based on transcriptome data,SSR locus prediction and codon usage bias analysis were performed.The results showed that 10083 unigenes were involved in 132 KEGG metabolic pathways,of which the metabolic pathway and the biosynthesis pathway of secondary metabolites were the two most abundant pathways.There were 13206 SSR loci in 9721 unigene sequences and 1115 transcription factors were predicted to belong to 54 families.In addition,the codon usage bias of color calla was weak,and the high frequency codons were AGG,CAG and AAG.

关 键 词：彩色马蹄莲 基因注释 功能分类 密码子使用偏好性 

分 类 号：S682.2[农业科学—观赏园艺]