利用SNP分子标记构建50份糯玉米自交系DNA指纹库  

Construction of DNA fingerprint database for 50 waxy corn inbred lines by SNP markers

作　　者：韩晴[1] 孙小淋 卢媛[1] 田东 施标[1] 沈雪芳[1] HAN Qing;SUN Xiaolin;LU Yuan;TIAN Dong;SHI Biao;SHEN Xuefang(Crog Breeding and Cultuation Research Institute,Shanghai Academy of Agrwultural Sciences,CIMMYT:China Sedalty Maize Research Center,Shanghai 201403,China;Ecc-EnHronmental Protectior Research Institute,Shanghai Academy of Agricultural Scienes,Shanghai 201403,China)

机构地区：[1]上海市农业科学院作物育种栽培研究所,CIMMYT:中国特用玉米研究中心,上海201403 [2]上海市农业科学院生态环境保护研究所,上海201403

出　　处：《上海农业学报》2020年第3期15-19,共5页Acta Agriculturae Shanghai

基　　金：上海市市级农口系统青年人才成长计划[沪农青字(2018)第1-6号];上海市鲜食玉米产业技术体系[沪农科产字(2019)第10号];上海市科学技术委员会科研计划项目(17391902702、153919N1300);上海市农业科学院卓越团队项目(农科创2017B-08);2019上海市农业科学院青年科技人员“助跑”计划;上海市科技支撑项目(17391900100);科技部重点研发项目(2018YFD0100106)。

摘　　要：为构建糯玉米品种DNA指纹库,保护品种资源,利用Illumina公司研发的包含56110个SNP标记的芯片,对50份糯玉米自交系进行基因分型。结果表明:按照SNP标记选用标准,共得到42406个SNP标记。这些标记的杂合率均值为0.04,SNP缺失率均值为0.01,最小等位基因频率(MAF)均值为0.4,多态性信息含量(PIC)均值为0.38,基因多样性指数均值为0.44。对上述获得的SNP标记设置不同数量位点进行筛选,确定25000位点为核心标记位点,可用于构建糯玉米自交系DNA指纹库。在25000位点时,对50份糯玉米自交系进行SNP聚类分析,共将其划分为四大类群,聚类结果与品种系谱来源一致。该研究可为糯玉米品种特异性和一致性的鉴定分析及品种保护工作提供理论指导。In order to construct the DNA fingerprint database of waxy corn varieties and protect the variety resources,50 waxy corn inbred lines were genotyped using a chip containing 56110 SNP(single nucleotide polymorphism)markers developed by Illumina.According to the selection criteria of SNP marker,a total of42406 SNP markers were obtained.The average heterozygosity rate was 0.04,the average SNP loss rate was 0.01,the average Minor Allelle Frequency(MAF)was 0.4,the average Polymorphic Information Content(PIC)was 0.38,the average gene diversity index was 0.44.Further,the number of core loci was screened by gradients analysis,and 25000 loci were optimal core loci for constructing fingerprinting of waxy corn inbred lines.Four groups were divided from the 50 inbred lines by cluster analysis,which was consistent with the pedigree.This study could provide guidances for the specificity,consistency of waxy corn varieties and varieties protection.

关 键 词：糯玉米 自交系 DNA指纹库 SNP标记 

分 类 号：S513[农业科学—作物学]