Fas/FasL途径在氟暴露致PC12细胞凋亡中的作用  

The role of Fas/FasL pathway in apoptosis of PC12 cells induced by fluoride exposure

作　　者：张佳勇 唐乐 于秋丽 阮琴[2] 章子贵[2] ZHANG Jia-yong;TANG Le;YU Qiu-li;RUAN Qin;ZHANG Zi-gui(College of Chemistry and Life Science,Zhejiang Normal University,Jinhua 321004,China;College of Xing Zhi,Zhejiang Normal University,Jinhua 321004,China)

机构地区：[1]浙江师范大学化学与生命科学学院,浙江金华321004 [2]浙江师范大学行知学院,浙江金华321004

出　　处：《中国环境科学》2020年第6期2700-2707,共8页China Environmental Science

基　　金：国家自然科学基金资助项目(81573101)。

摘　　要：为了探讨Fas/FasL途径在氟暴露致PC12细胞凋亡中的作用及其机制,采用含20、40、80、160mg/L NaF培养液处理PC12细胞.结果表明,所有剂量Na F处理12、24、36、48h,PC12细胞活性升高;上述不同剂量Na F处理24h后,与对照组比,PC12细胞的活性氧水平、细胞凋亡率、细胞内Fas/FasL信号转导通路Fas和Fas L、Caspase8、FADD、Caspase3基因和蛋白表达水平均呈显著上升(P<0.05),而Bid基因和蛋白表达水平显著下降(P<0.05),且呈氟暴露剂量依赖性.结果提示Fas/FasL途径在氟暴露致PC12细胞凋亡中起重要作用,其中FADD可能是Fas/FasL凋亡途径中的重要靶分子.The present study investigated the effects of exposure to fluoride on apoptosis of PC12 cells and aimed to clarify the role of Fas/FasL pathway involved in this effect.The results showed that treatment with NaF at 20,40,80,160mg/L for 12,24,36,and 48hours enhanced the activity of PC12 cells,respectively.Treatment with all dosages of NaF for 24hours significantly increased the levels of reactive oxygen species(ROS)and apoptosis rate and dose-dependently up-regulated the gene/protein expression levels of Fas,FasL,Caspase8,FADD,and Caspase3,but down-regulated the gene/protein expression levels of Bid(P<0.05).These results suggest that Fas/FasL apoptotic pathway plays an important role in the apoptosis of PC12 cells induced by fluoride exposure,in which FADD may be an important target molecule of fluoride exposure-induced apoptosis.

关 键 词：氟暴露 Fas/FasL凋亡途径 PC12细胞 细胞凋亡 

分 类 号：X171.5[环境科学与工程—环境科学]