PPARγ基因多态性与中国北方人群散发性帕金森病相关性分析  

ASSOCIATION OF PPARγ GENE POLYMORPHISMS WITH SPORADIC PARKINSON’S DISEASE IN THE NORTHERN CHINESE POPULATION

作　　者：李珊珊[1] 王慧敏[1] 金佳宁 白新玲 谢安木[1] LI Shanshan;WANG Huimin;JIN Jianing;BAI Xinling;XIE Anmu(Department of Neurology, The Affiliated Hospital of Qingdao University, Qingdao 266003, China)

机构地区：[1]青岛大学附属医院神经内科,山东青岛266003

出　　处：《精准医学杂志》2020年第3期237-240,共4页Journal of Precision Medicine

基　　金：国家自然科学基金项目(81571225)。

摘　　要：目的探讨过氧化物酶体增殖物激活受体γ(PPARγ)基因多态性与中国北方人群散发性帕金森病(PD)的相关性。方法选取我院391例确诊为PD的患者(PD组)和体检中心391例与PD组性别、年龄相匹配的健康受试者(对照组),采用聚合酶链式反应(PCR)和限制性片段长度多态性(RFLP)方法检测两组研究对象rs3856806和rs1801282位点的基因分型。同时再按照年龄和性别进行亚组分组,PD组中发病年龄≤50岁者为早发性PD组(B1组),>50岁者为晚发性PD组(B2组);B1组和B2组再分别按照性别分为男B1组(B1-1组)、女B1组(B1-2组)、男B2组(B2-1组)及女B2组(B2-2组);对照组按照同样的分组方法进行分组,分为A1~2组及A1-1~A2-2组。结果B1组与A1组、B1-1组与A1-1组、B1-2组与A1-2组以及B2组与A2组、B2-1组与A2-1组、B2-2组与A2-2组PPARγ基因rs3856806位点基因型和等位基因频率比较,差异无显著意义(P>0.05)。B1-2组与A1-2组、B2组与A2组、B2-1组与A2-1组、B2-2组与A2-2组PPARγ基因rs1801282位点基因型和等位基因频率比较,差异无显著性(P>0.05);B1组与A1组、B1-1组与A1-1组PPARγ基因rs1801282位点基因型比较,差异具有显著性(χ^2=8.419,8.090,P<0.05);且B1组及B1-1组的G等位基因频率分别高于A1组及A1-1组(χ^2=7.953,P<0.05,OR=11.170,95%CI=1.403~88.911;χ^2=7.630,P<0.05,OR=1.123,95%CI=1.030~1.223)。两位点不同单体型分布在PD组和对照组间未发现显著统计学差异(P>0.05)。结论PPARγ基因rs1801282位点等位基因G可能会增加中国北方人群早发性PD的患病风险。Objective To investigate the association of peroxisome proliferator-activated receptor gamma(PPARγ)gene polymorphisms and susceptibility to Parkinson’s disease(PD)in the northern Chinese population.Methods We enrolled 391 outpatients with PD(PD group)and 391 healthy participants(control group)matched for age and sex from the physical examination center in the Affiliated Hospital of Qingdao University.According to the age of onset,the patients were divided into group B1(age of onset≤50 years,defined as early-onset PD)and group B2(age of onset>50 years,defined as late-onset PD).They were further divided by sex into group B1-1(the male in group B1),group B1-2(the female in group B1),group B2-1(the male in group B2),and group B2-2(the female in group B2).The control group was divided in the same way into group A1,group A1-1,group A1-2,group A2,group A2-1,and group A2-2.The genotypes of rs1801282 and rs3856806 in the PPARγgene were determined for all the subjects by polymerase chain reaction and restriction fragment length polymorphism analysis.Results There were no significant differences in the genotype and allele frequencies of rs3856806 between groups B1 and A1,between groups B1-1 and A1-1,between groups B1-2 and A1-2,between groups B2 and A2,between groups B2-1 and A2-1,or between groups B2-2 and A2-2(all P>0.05).In terms of the genotype and allele frequencies of rs1801282,no significant differences were observed between groups B1-2 and A1-2,between groups B2 and A2,between groups B2-1 and A2-1,or between groups B2-2 and A2-2(all P>0.05);but the genotype frequency showed significant differences between groups B1 and A1 and between groups B1-1 and A1-1(χ^2=8.419,8.090,P<0.05),and the frequency of G allele was significantly higher in group B1 than in group A1(χ^2=7.953,P<0.05,OR=11.170,95%CI=1.403-88.911),and significantly higher in group B1-1 than in group A1-1(χ^2=7.630,P<0.05,OR=1.123,95%CI=1.030-1.223).No significant difference was found between the PD group and the control group in the haplotype distr

关 键 词：PPARΓ 多态性 单核苷酸 帕金森病 基因型 北部地方 中国 

分 类 号：R742.5[医药卫生—神经病学与精神病学] R394.5[医药卫生—临床医学]