miRNA-184在胶质瘤组织中的表达及其对胶质瘤细胞增殖和凋亡的影响  

Expression of miRNA-184 in glioma tissues and its effect on glioma cells proliferation and apoptosis

作　　者：李永红[1,2] 刘伟仪 李伟 彭文[1] 董链 杨峥嵘 刘文明 郭蒸[2] Li Yonghong;Liu Weiyi;Li Wei;Peng Wen;Dong Lian;Yang Zhengrong;Liu Wenming;Guo Zheng(Department of Oncology,Tianmen First People's Hospital,Hubei Tianmen 431700,China;Gannan Medical College,Jiangxi Ganzhou 341000,China;Department of Oncology,Tongling People's Hospital,Anhui Tongling 244000,China)

机构地区：[1]天门市第一人民医院肿瘤科,湖北天门431700 [2]赣南医学院,江西赣州341000 [3]铜陵市人民医院肿瘤科,安徽铜陵244000

出　　处：《现代肿瘤医学》2020年第14期2385-2389,共5页Journal of Modern Oncology

基　　金：国家自然科学基金资助项目(编号:81860440);湖北省卫生健康科研基金资助项目(编号:WJ2019H211)。

摘　　要：目的:探讨miRNA-184在胶质瘤组织中的表达及其对胶质瘤细胞增殖和凋亡的影响以及胶质瘤发生发展过程中的分子机制。方法:利用荧光定量PCR(RT-qPCR)检测miRNA-184在患者胶质瘤组织以及癌旁组织中的表达水平;利用体外细胞培养实验向胶质瘤细胞中转染miRNA-184 mimics;利用CCK-8比色法和流式细胞仪分别检测转染miRNA-184 mimics后胶质瘤细胞的增殖能力和凋亡情况;利用Western blot检测转染miRNA-184 mimics后胶质瘤细胞中AKT蛋白的表达水平。结果:荧光定量PCR检测结果表明,miRNA-184在患者胶质瘤组织中的表达水平显著低于癌旁组织(P<0.05);细胞体外转染实验结果表明,转染miRNA-184 mimics后,胶质瘤细胞的增殖能力显著下降,凋亡率显著升高(P<0.01);Western blot检测结果表明,转染miRNA-184 mimics后胶质瘤细胞中AKT蛋白表达水平显著下降(P<0.05)。结论:miRNA-184在胶质瘤组织中显著低表达,miRNA-184与胶质瘤的发生发展密切相关;过表达miRNA-184可以抑制胶质瘤细胞的增殖,促进胶质瘤细胞的凋亡;miRNA-184可能通过抑制AKT信号通路从而抑制胶质瘤的发生发展。Objective:To investigate the relationship between miRNA-184 in glioma tissues and its effect on glioma cell proliferation and apoptosis,and the molecular mechanism of the occurrence and development of glioma.Methods:RT-qPCR was used to detect the expression level of miRNA-184 in glioma tissues and adjacent tissues.miRNA-184 mimics was transferred into glioma cells by vitro cell culture assay.CCK-8 colorimetric assay and flow cytometry were used to detect the proliferation and apoptosis of glioma cells transfected with miRNA-184 mimics,respectively.Western blot was used to detect the expression level of AKT protein in glioma cells transfected with miRNA-184 mimics.Results:The results of RT-qPCR showed that the expression level of miRNA-184 in human glioma tissues was significantly lower than that in adjacent tissues(P<0.05).The results of vitro transfection experiments showed that the proliferation ability of glioma cells decreased significantly and the apoptosis rate increased significantly after miRNA-184 mimics transfer(P<0.01).The Western blot assay showed that the expression level of AKT protein in glioma cells after miRNA-184 mimics transfer was significantly decreased(P<0.05).Conclusion:The expression level of miRNA-184 is significantly downregulated in glioma tissues and miRNA-184 is closely related to the occurrence and development of glioma.Overexpression of miRNA-184 can inhibit the proliferation of glioma cells and promote the apoptosis of glioma cells.miRNA-184 inhibits the occurrence and development of glioma by inhibiting the AKT signaling pathway.

关 键 词：miRNA-184 胶质瘤 AKT蛋白 

分 类 号：R730.264[医药卫生—肿瘤]