数字PCR及荧光定量PCR测定豆乳饮料转基因成分分析  

Determination of Transgenic Components in Soybean Milk Beverage by Digital PCR and Fluorescence Quantitative PCR

作　　者：张彦斌 景利斌 高智慧 赵晓娜 张宏博 Zhang Yanbin;Jing Libin;Gao Zhihui;Zhao Xiaona;Zhang Hongbo(Inner Mongolia Autonomous Region Food Inspection and Testing Center,Hohhot 010090,China)

机构地区：[1]内蒙古自治区食品检验检测中心,内蒙古呼和浩特010090

出　　处：《现代食品》2020年第9期191-193,198,共4页Modern Food

基　　金：内蒙古自治区科技重大专项(编号:ZDZX2016016);内蒙古自治区科技创新引导奖励资金项目(2017年度);内蒙古自治区科技创新引导奖励资金项目(编号:KCBJ2018068)。

摘　　要：研究将转基因启动子CaMV35s、终止子NOS基因作为靶标,内标选择大豆内源基因Lectin,分别采用微滴式数字PCR及实时荧光PCR对标准品转基因大豆进行检测,实测20批次豆乳饮料转基因成份。结果显示,与实时荧光PCR相比,微滴式数字PCR对转基因筛查浓度检测低限、含量低限均较低,分别为0.04 ng/反应、0.05%,与欧盟转基因标识阈值要求符合。20批次样品中有16批次经过不同平台检测结果为阴性,1批次为阳性,3批次经过数字PCR检测获得准确结果,表明该检测技术能对实时荧光PCR起到辅助检测作用。In this study,the transgenic promoter CaMV35s and terminator NOS gene were used as targets,the endogenous gene Lectin of soybean was selected as internal standard,the standard transgenic soybean was detected by microdroplet digital PCR and real-time fluorescence PCR respectively,and the transgenic components of 20 batches of soybean milk beverage were measured.The results showed that compared with real-time fluorescence PCR,the detection limit and content limit of microdroplet digital PCR for transgene screening concentration were lower,0.04 ng/reaction and 0.05%,respectively,which were in line with the requirements of EU GM labeling threshold.16 of the 20 batches of samples tested by different platforms were negative,1 batch was positive,and 3 batches were tested by digital PCR to obtain accurate results.It is suggested that the detection technology can play an auxiliary role in real-time fluorescence PCR.

关 键 词：微滴式数字PCR 实时荧光PCR 豆乳饮料 转基因成分 浓度检测 

分 类 号：TS214.2[轻工技术与工程—粮食、油脂及植物蛋白工程]