茶多酚对周围神经损伤的修复作用研究  

Effects of tea polyphenol on peripheral nerve injury and repair

作　　者：汤银娟 陈龙 段彦之 李姝 朱斌 左志敏 王祥海[3] 李振林[3] TANG Yin-juan;CHEN Long;DUAN Yan-zhi;LI Shu;ZHU Bin;ZUO Zhi-min;WANG Xiang-hai;LI Zhen-lin(School of Basic Medical Sciences,Xiangnan University,Chenzhou 423000,Hunan,China;School of Clinical Medicine,Xiangnan University,Chenzhou 423000,Hunan,China;School of Basic Medical Sciences,Southern Medical University,Guangzhou 510515,China)

机构地区：[1]湘南学院基础医学院,湖南郴州423000 [2]湘南学院临床学院,湖南郴州423000 [3]南方医科大学,广州510515

出　　处：《中国临床解剖学杂志》2020年第3期301-307,313,共8页Chinese Journal of Clinical Anatomy

基　　金：湖南省教育厅优秀青年项目(16B245);郴州市科技项目(CZ-XNXY201509);大学生研究性学习与创新性实验项目(湘南学院校发[2018]147号)。

摘　　要：目的研究茶多酚促进周围神经损伤修复的效果。方法成年BALB/c小鼠48只,随机分为茶多酚治疗组、生理盐水组及假手术组,每组16只。前两组制备右侧坐骨神经夹伤模型后每天以灌胃法给药,假手术组仅暴露右侧坐骨神经但不做其它处理。术后14 d通过足印分析检测小鼠患肢的运动功能,针刺法检测感觉功能;随后每组6只小鼠取坐骨神经提取RNA进行qPCR检测,其余10只小鼠的坐骨神经用于制备NF-200和MBP免疫组化显示再生轴突与髓鞘;其腓肠肌称湿重后制备HE染色切片。结果相对于生理盐水组,茶多酚治疗组的运动及感觉功能检测指标均有改善;腓肠肌湿重比及肌纤维平均横截面积的提高具有统计学意义;损伤神经的远端再生轴突及髓鞘也有提高。结论茶多酚能改善由神经损伤所致的行为学功能丧失以及神经病理改变。Objective To explore the effects of tea polyphenol(TP)on peripheral nerve injury and repair.Methods 48 adult BALB/c mice were randomly divided into three groups:a TP treatment group,a saline control group and a sham group.The right sciatic nerve of each mouse in the TP treatment group and the saline control group was injured by crush and then the mice were intragastrically administrated with TP or saline twice per day for successive 14 days.The mice in sham group were only exposed their right sciatic nerve without other treatment.The motor function of each mouse was detected by footprint assay and the sensory function was evaluated with acupuncture assessment 14 days after the surgery.Subsequently,the sciatic nerves of 6 mice in each group were collected for qPCR assay.The other 10 mice of each group were subjected to perform the NF-200/MBP double immunostaining to show the regenerated axons and myelin in the distal trunk of the injured nerves.The gastrocnemius muscle was weighed and then to prepare the HE stained section.Results Compared with the saline control group,the TP treatment resulted in significantly improved functions of motor and sensory,higher wet weight ratio of gastrocnemius muscle and bigger area size of cross-section of myofibers,as well as more regenerated axons and myelin in the injured nerves.Conclusions This study show that tea polyphenols could improve the behavioral functional loss and neuropathologic changes caused by nerve injury.

关 键 词：茶多酚 周围神经损伤 神经再生 

分 类 号：R322.85[医药卫生—人体解剖和组织胚胎学]