解郁丸对WKY抑郁样大鼠前额叶皮层与海马树突棘的影响  

Effect of Jieyuwan on dendritic spines in prefrontal cortex and hippo⁃campus in WKY depression-like rats

作　　者：刘红梅 李琦[2] 宋苗 张宇[1] 徐勇[2] LIU Hong-mei;LI Qi;SONG Miao;ZHANG Yu;XU Yong(Basic Medical College,First Hospital/First Clinical Medical College,Shanxi Medical Universi⁃ty,Taiyuan 030001,China;Department of Psychiatry,First Hospital/First Clinical Medical College,Shanxi Medical Universi⁃ty,Taiyuan 030001,China)

机构地区：[1]山西医科大学基础医学院,山西太原030001 [2]山西医科大学第一医院精神卫生科,山西太原030001

出　　处：《中国病理生理杂志》2020年第6期1049-1054,共6页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.81571319);认知障碍多学科诊疗山西省科技创新团队计划(No.201705D131027);国家重点研究与发展项目(No.2016YFC1307004);三晋学者;山西省136兴医工程项目。

摘　　要：目的:观察Wistar-Kyoto(WKY)抑郁模型大鼠前额叶皮层与海马组织中树突棘的变化,并探讨解郁丸在其中的作用。方法:以成年雄性WKY大鼠为实验组,选用同品系SPF级Wistar大鼠为其对照组。首先,用糖水偏好实验、旷场实验和强迫游泳实验检测大鼠行为学变化,并作为其基线;然后将实验组所有WKY大鼠随机分为模型组、解郁丸组和西酞普兰组,与对照组分别进行21 d灌胃给药,再用相同的行为学方法检测给药后行为的变化。采用Golgi染色观察前额叶皮层与海马组织中树突棘的病理特点。Western blot检测前额叶皮层与海马组织突触后致密区蛋白95(PSD-95)的表达水平。结果:给药前,WKY大鼠表现出明显的抑郁样行为,前额叶皮层与海马组织中树突棘的密度明显降低(P<0. 01),且PSD-95蛋白的表达水平明显降低(P<0. 01);经药物治疗后,WKY大鼠的抑郁样行为明显减少,前额叶皮层与海马组织中树突棘的密度升高(P<0. 01),PSD-95蛋白的表达水平也提高(P<0. 01)。结论:解郁丸能明显减少WKY大鼠抑郁样行为,且影响树突棘的结构改变及PSD-95的蛋白表达。树突棘可能是抑郁症早期改变的重要结构之一。AIM:To observe the changes of dendritic spines in prefrontal cortex and hippocampus of Wistar-Kyoto(WKY)depression-like rats,and to explore the effects of Jieyuwan(JYW)on them.METHODS:The male WKY rats were selected as the experimental group,and the same strain of Wistar rats were selected as the control group.Firstly,sucrose preference test,open-field experiment and forced swimming test were used to detect the behavior changes in the rats as their baseline.Then,all WKY rats were randomly divided into model(WKY+NaCl)group,WKY+JYW group and WKY+citalopram group.All WKY rats and Wistar rats(Wistar+NaCl group)were administered intragastrically for 21 d,and the changes of behavior after administration were detected by the same behavioral methods.Golgi staining was used to observe the pathological characteristics of dendritic spines in the prefrontal cortex and hippocampus,and Western blot was used to detect the protein expression level of postsynaptic density protein-95(PSD-95)in the prefrontal cortex and hippo⁃campus.RESULTS:Before administration,WKY rats clearly showed depression-like behavior,the density of dendritic spines in the prefrontal cortex and hippocampus decreased significantly(P<0.01),and the protein expression level of PSD-95 was significantly reduced(P<0.01).After treatment with the drugs,the depression-like behavior of WKY rats was significantly attenuated,the density of dendritic spines in the prefrontal cortex and hippocampus increased(P<0.01),and the protein expression level of PSD-95 also increased(P<0.01).CONCLUSION:Jieyuwan significantly at⁃tenuates the depression-like behavior of WKY rats,and affects the structural changes of dendritic spines and the expression of PSD-95 protein,which further proves that dendritic spines may be one of the importantearly structural changes in depression.

关 键 词：抑郁症 解郁丸 前额叶皮层 海马 树突棘 

分 类 号：R749.4[医药卫生—神经病学与精神病学] R363.2[医药卫生—临床医学]