长链非编码RNA PSMA3-AS1在人胶质瘤细胞中的表达及作用研究  

The expression and role of LncRNA PSMA3-AS1 in human glioma cells

作　　者：张遥[1] 范丹 ZHANG Yao;FAN Dan(Department of Neurosurgery,The Second Affiliated Hospital of Harbin Medical University,Harbin 150001,China;Department of Ophthalmology,The Second Hospital of Heilongjiang Province)

机构地区：[1]哈尔滨医科大学附属第二医院神经外科,哈尔滨150001 [2]黑龙江省第二医院眼科

出　　处：《实用肿瘤学杂志》2020年第3期214-219,共6页Practical Oncology Journal

摘　　要：目的探究胶质瘤组织中lncRNA PSMA3-AS1的表达情况,以及其对胶质瘤细胞(U251、LN229、T98G和A172)增殖和侵袭能力的影响。方法使用qRT-PCR实验检测35对胶质母细胞瘤组织和癌旁组织中PSMA3-AS1表达;结合TCGA数据库分析PSMA3-AS1表达与胶质瘤恶性程度及患者预后的关系;采用U251和T98G胶质瘤细胞系进行细胞增殖能力和侵袭能力检测;使用Western blot实验检测侵袭相关蛋白MMP-2/9表达水平。结果TCGA数据库分析提示PSMA3-AS1在胶质瘤组织中呈高表达状态,结合35对胶质瘤组织和细胞系中PSMA3-AS1的表达,提示PSMA3-AS1在胶质瘤中表达明显上调(P<0.05)。同时发现PSMA3-AS1表达与肿瘤直径有关(P=0.007),而PSMA3-AS1高表达的胶质瘤患者生存期较短(P=0.042)。敲低PSMA3-AS1表达后,胶质瘤细胞内PSMA3-AS1的表达水平及细胞增殖、侵袭能力明显受到抑制,侵袭相关蛋白(MMP-2/9)表达水平明显降低(P<0.05)。结论PSMA3-AS1在胶质瘤中呈高表达状态,具有促癌作用,可能是潜在的胶质瘤治疗靶点。Objective The aim of this study was to detect the expression of lncRNA PSMA3-AS1 in glioma tissues and cell lines(U251、LN229、T98G and A172)and its effect on glioma cells.Methods Quantitative qRT-PCR method was used to evaluate the expression of PSMA3-AS1 in 35 glioma tissues;Combined with TCGA databases,the correlation between PSMA3-AS1 expression and the degree of malignancy and the prognosis of glioma patients was analyzed;Cell proliferation and invasion were explored in U251 and T98G glioma cells;The effects of PSMA3-AS1 on invasion related proteins MMP-2/9 in glioma cells were detected by Western blot assay.Results TCGA database indicated that PSMA3-AS1 was increased in glioma tissues,and the expression of PSMA3-AS1 in 35 paired glioma tissues and glioma cells was significantly increased than in adjacent normal brain tissues(P<0.05).The expression of PSMA3-AS1 was correlated with tumor mean diameter(P=0.007),and high PSMA3-AS1 exhibited poorer overall survival in glioma patients(P=0.042).After knockdown the level of PSMA3-AS1,the proliferation and invasion abilities of glioma cells were significantly inhibited and the expression of MMP-2/MMP9 were decreased in glioma cells(P<0.05).Conclusion The level of PSMA3-AS1 is significantly higher in glioma tissues with a role of promoting cancer in glioma and may be a potential therapeutic target.

关 键 词：lncRNA 胶质瘤 细胞增殖 侵袭 

分 类 号：R739.41[医药卫生—肿瘤]