长链非编码RNA PVT1通过miR-17-5p调控肝癌细胞增殖与迁移的机制研究  

Long non-coding RNA PVT1 regulates cell proliferation and metastasis through miR-17-5p in hepatocellular carcinoma

作　　者：崔士猛 杜渐[1] 罗海峰[1] 闻庆平[2] 苗壮[2] 温超[2] 吴越[2] Cui Shimeng;Du Jian;Luo Haifeng(Dept of General Surgery,The First Affiliated Hospital of Dalian Medical University,Dalian 116011)

机构地区：[1]大连医科大学附属第一医院普外科,大连116011 [2]大连医科大学附属第一医院麻醉科,大连116011

出　　处：《安徽医科大学学报》2020年第6期932-936,共5页Acta Universitatis Medicinalis Anhui

基　　金：辽宁省自然科学基金(编号:20170540266)。

摘　　要：目的探讨肿瘤调控因子长链非编码RNA(lncRNA) PVT1对肝癌(HCC)细胞增殖与迁移能力的影响及机制。方法通过TCGA数据库对HCC中PVT1的表达水平进行分析。采用瞬时转染技术构建PVT1敲低细胞系。通过CCK-8实验及划痕实验检测PVT1对HCC细胞增殖与迁移能力的影响。采用生物信息预测和荧光素酶实验验证PVT1与miR-17-5p的靶向结合情况。利用共转染实验验证PVT1能够通过miR-17-5p实现对HCC细胞增殖与迁移的调控。结果 PVT1在HCC组织和细胞中高表达。敲低PVT1后HCC细胞增殖与迁移能力显著降低。荧光素酶实验证实PVT1能够与miR-17-5p靶向结合,并且miR-17-5p介导了PVT1对HCC细胞增殖和迁移能力的调控作用。结论 lncRNA PVT1能够通过靶向抑制miR-17-5p从而促进HCC细胞的增殖和迁移。Objective To elucidate the role of long non-coding RNA(lncRNA),a novel tumor regulator,PVT1 in hepatocellular carcinoma(HCC).Methods According to the analysis of TCGA database,PVT1 was highly expressed in HCC.Transient transfection was used to construct PVT1 knockdown cell lines.CCK-8 assay and scratch assay were used to evaluate the ability of cell growth and metastasis.Bioinformatics prediction and luciferase assay were used to validate the targeted binding of PVT1 to miR-17-5 p.The co-transfection experiment was used to verify the effect of PVT1 on proliferation and metastasis through miR-17-5 p.Results The results showed that PVT1 was highly expressed in HCC tissues and cell lines.Knockdown of PVT1 decreased the ability of cells proliferation and metastasis.Luciferase assay confirmed that PVT1 could bind to miR-17-5 p,and miR-17-5 p mediated the effect of PVT1 on the proliferation and metastasis of HCC cells.Conclusion lncRNA PVT1 can enhance the proliferation and metastasis of HCC cells through miR-17-5 p.The above results indicate the important role of PVT1 in HCC and provide a good prospect for future targeted therapy.

关 键 词：PVT1 miR-17-5p 肝癌 迁移 增殖 

分 类 号：R73[医药卫生—肿瘤]