TIMP-4和MMP-2在胃癌中的表达  

Expression of TIMP-4,MMP-2 in gastric cancer

作　　者：孔晋 吴文涌[1] 吴正升[2] Kong Jin;Wu Wenyong;Wu Zhengsheng(Dept of General Surgery,The First Affiliated Hospital of Anhui Medical University,Hefei 230022;Dept of Pathology,Anhui Medical University,Hefei 230032)

机构地区：[1]安徽医科大学第一附属医院普外科,合肥230022 [2]安徽医科大学病理学教研室,合肥230032

出　　处：《安徽医科大学学报》2020年第6期965-969,共5页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81572305)。

摘　　要：目的分析金属蛋白酶组织抑制因子-4(TIMP-4)及基质金属蛋白酶-2(MMP-2)在胃癌(GC)及癌旁非肿瘤组织中的表达,并探讨其在胃癌中的临床意义。方法采用免疫组织化学法分析75例胃腺癌组织及42例癌旁非肿瘤组织中TIMP-4蛋白和MMP-2蛋白表达水平,同时采用Pearson相关分析法分析TIMP-4蛋白和MMP-2蛋白之间的相关性。TCGA数据库中Kaplan-Meier生存分析胃癌中TIMP-4和MMP-2的高表达与患者总生存期的相关性。结果 75例GC组织中,TIMP-4蛋白阳性65例(86.67%),MMP-2蛋白阳性68例(90.67%),癌旁非肿瘤组织TIMP-4阳性22例(52.38%),癌旁非肿瘤组织MMP-2阳性23例(54.76%),癌组织与癌旁非肿瘤组织相比差异均有统计学意义(P<0.01);MMP-2蛋白的表达与肿瘤与淋巴结转移及胃癌分期相关且差异有统计学意义(P<0.001);但与性别、年龄、肿瘤大小以及分化程度无统计学相关。TIMP-4蛋白表达与性别、年龄、肿瘤大小、肿瘤淋巴结转移、肿瘤分化程度及分期均无关联(P>0.05)。Pearson相关分析表明TIMP-4蛋白和MMP-2蛋白表达呈正相关(r=0.351,P<0.001)。Kaplan-Meier生存分析显示TIMP-4、MMP-2高表达的胃癌患者预后较差。结论 TIMP-4和MMP-2蛋白均在胃癌组织中高表达,其高表达均提示胃癌患者预后较差,可能共同参与胃癌的发生发展,有望成为预测胃癌转移的潜在生物学标志物。Objective To analyze the expression of tissue inhibitor of metalloproteinase-4(TIMP-4)and matrix metalloproteinase-2(MMP-2)in gastric cancer(GC)and adjacent tissues,and to explore their clinical significance in GC.Methods The expression levels of TIMP-4 protein and MMP-2 protein in 75 cases of gastric adenocarcino-ma tissues and 42 adjacent tissues were analyzed by immunohistochemical method,and the correlation betweenTIMP-4 protein and MMP-2 protein was analyzed by Pearson correlation analysis.Kaplan-meier survival analysis of GC in TCGA was performed to analyze the correlation between high TIMP-4 and MMP-2 expression in GC and overall survival.Results Among 75 GC tissues,65 cases were positive for TIMP-4 protein(86.67%),68 cases were positive for MMP-2 protein(90.67%),and 22 cases were positive for TIMP-4 in non-tumor tissues adjacent to cancer(52.38%),23 cases of MMP-2 positive in non-tumor tissues adjacent to cancer(54.76%).The differences were statistically significant compared with adjacent tissues(P<0.01).The expression of MMP-2 protein was statistically related to lymph node metastasis(P<0.001)and cancer stage(P<0.05),but it was not statistically related to gender,age,tumor size or degree of differentiation.There was no statistical correlation between TIMP-4 protein expression,gender,age,tumor size,lymph node metastasis,tumor differentiation or cancer stage(P>0.05).Pearson correlation analysis found a positive correlation between MMP-2 protein and TIMP-4 protein expression(r=0.351,P<0.001).Kaplan-meier survival analysis showed that GC patients with high expression of TIMP-4 and MMP-2 had a poor prognosis.Conclusion MMP-2 protein and TIMP-4 protein are highly expressed in GC tissues,and the high expression suggest poor prognosis of GC patients,thus which may jointly participate in the occurrence and development of GC andwere expected to become a potential biological marker for predicting metastasis of GC.

关 键 词：金属蛋白酶组织抑制因子-4 基质金属蛋白酶-2 胃癌 免疫组化 

分 类 号：R735.2[医药卫生—肿瘤]