人卵巢浆液性腺癌原代细胞的改良体外培养方法  

A modified in vitro primary culture method of human ovarian serous adenocarcinoma cells

作　　者：张晨晨 蔡泽宇 王弦[1] 江冬瑞[1] 吴强[1,3] 王晶[4] Zhang Chenchen;Cai Zeyu;Wang Xian(Dept of Pathology,The Second Affiliated Hospital of Anhui Medical University,Hefei 230601;Dept of Pathology,School of Basic Medical Sciences,Anhui Medical University,Hefei 230032)

机构地区：[1]安徽医科大学第二附属医院病理科,合肥230601 [2]安徽医科大学基础医学院病理教研室,合肥230032 [3]安徽医科大学第一附属医院病理科,合肥230022 [4]安徽医科大学第一附属医院妇产科,合肥230022

出　　处：《安徽医科大学学报》2020年第6期979-983,共5页Acta Universitatis Medicinalis Anhui

基　　金：安徽省转化医学研究院科研基金(编号:2017zhyx36)。

摘　　要：通过对常见的人卵巢浆液性腺癌的原代细胞(HOSACs)培养方法进行改良,建立一种简单高效的HOSACs培养方法。收集25例临床病理确诊为卵巢浆液性腺癌组织标本,每个标本随机分成大小相等的改良组和传统组,改良组用改良酶消化法结合组织贴壁法来提取细胞(胰蛋白酶+透明质酸酶消化2~3 min+组织块与细胞混悬液铺板);传统组用传统的组织块贴壁法(组织块剪碎后直接铺板)来提取细胞。通过细胞免疫荧光和CCK-8法比较2组培养方法获得的细胞纯度与细胞活性;并在相差显微镜下观察2组细胞分别在48 h和72 h的形态特征。免疫荧光和CCK-8结果显示改良组获得的细胞数量、细胞活性以及细胞纯度均高于传统组(P<0.05)。A simple and efficient method of human ovarian serous adenocarcinoma cells(HOSACs),culture was established through improving methods for culturing primary cells of the common human ovarian serous adenocarcinoma.25 tissue specimens of serous ovarian carcinoma confirmed by clinicopathology were collected,and each specimen was randomly divided into two groups:the modified group and the traditional group.The modified group was extracted cells with modified enzyme digestion method and tissue block adherence method(trypsin+hyaluronidase digestion for 2~3 min+tissue block and cell suspension plating).Traditional group was extracted with tissue block adherence method(the tissue block is shredded directly and then plating).The cell purity and cell viability obtained by the two methods were compared by cell immunofluorescence and CCK-8.The growth characteristics of primary cells of the two groups were observed under phase contrast microscope at 48 h and 72 h.The results of immunofluorescence and CCK-8 showed that the number of cells,cell viability and cell purity obtained in the modified group were higher than those in the traditional group(P<0.05).

关 键 词：卵巢浆液性腺癌 人卵巢癌原代细胞 原代培养 细胞鉴定 

分 类 号：R737[医药卫生—肿瘤]