生姜提取液对常温物流甘薯软腐病的影响及其机制研究  

Effect of ginger extract on sweet potatoes with soft rot disease in room temperature logistics and its mechanism

作　　者：刘锦 张洪翠 程圣 何欣遥 张敏[1] LIU Jin;ZHANG Hongcui;CHENG Sheng;HE Xinyao;ZHANG Min(College of Food Science of Southwest University,Food Storage and Logistics Research Center of Southwest University,Laboratory of Quality Safety Risk Assessment for Argo-products on Storage and Preservation(Chongqing),Ministry of Agriculture,Chongqing 400715,China)

机构地区：[1]西南大学食品科学学院,西南大学食品贮藏与物流研究中心,农业部农产品贮藏保鲜质量安全风险评估实验室(重庆),重庆400715

出　　处：《食品与发酵工业》2020年第12期57-65,共9页Food and Fermentation Industries

基　　金：重庆市科委社会事业与民生保障科技创新专项(cstc2015shms-ztzx80010)。

摘　　要：该文使用体积分数为25%、50%、100%生姜提取液对软腐病致病菌匍枝根霉进行体外和甘薯活体实验,探究生姜提取液处理对常温物流条件下甘薯软腐病的控制作用,以解决甘薯常温物流腐烂问题。结果表明,体外培养试验中生姜提取液对匍枝根霉的菌丝生长、孢子萌发以及孢子芽管的生长均有抑制作用,且体积分数越高效果越好;活体实验中,生姜提取液处理能提高甘薯多酚氧化酶(polyphenol oxidase,PPO)、过氧化物酶(peroxidase,POD)、超氧化物歧化酶(superoxide dismutase,SOD)、苯丙氨酸解氨酶(phenylalamine ammonia-lyase,PAL)的活性,抑制过氧化氢酶(catalase,CAT)、抗坏血酸过氧化物酶(ascorbate peroxidase,APX)酶活,同时维持甘薯较高的总酚和类黄酮含量,有效降低了甘薯软腐病发病率。综上,生姜提取液不仅可以直接抑制匍枝根霉的生长,还能通过调节甘薯的生理变化抵御微生物的侵入,抑制软腐病的发生,且体积分数越高效果越好。Ginger extract with a volume fraction of 25%,50%and 100%was used to carry out in vitro and in vivo experiments on Rhizopus stolonifer,a pathogen of soft rot,to explore the effect of ginger extract on soft rot of sweet potatoes under room temperature logistics conditions,so as to solve the problem of soft rot of sweet potatoes under room temperature logistics conditions.The results showed that ginger extract could inhibit the mycelia growth,spore germination and spore bud tube growth of Rhizopus stolonifera in vitro culture test,and the higher the volume fraction,the better the effect.In vivo experiment,ginger extract can increase the activity of polyphenol oxidase(PPO),peroxidase(POD),superoxide dismutase(SOD),peroxidase(POD),superoxide dismutase(SOD)and phenylalamine ammonia-lyase(PAL)and inhibit the activity of catalase(CAT)and ascorbate peroxidase(APX)while maintaining the higher content of total phenol and flavonoids in sweet potatoes,which effectively reduced the incidence of soft rot in sweet potatoes.To sum up,ginger extract could not only directly inhibit the growth of Rhizopus stolonifer,but also resist the invasion of microorganisms by regulating the physiological changes of sweet potatoes,and inhibit the occurrence of soft rot,and the higher the volume fraction,the better the effect.

关 键 词：生姜提取液 匍枝根霉 甘薯软腐病 体外实验 甘薯活体实验 

分 类 号：S435.31[农业科学—农业昆虫与害虫防治]