颈7根性撕脱诱发神经病理性痛小鼠模型的研究  

A study on the model of neuropathic pain in mice induced by C7 root avulsion

作　　者：张凡亮 鲜航 梁超 罗层 丛锐 Zhang Fanliang;Xian Hang;Liang Chao;Luo Ceng;Cong Rui(Department of Orthopedics,the First Affiliated Hospital of the Air Force Medical University,Xi′an 710032,China;Department of Neurobiology and Collaborative Innovation Center for Brain Science,Basic Medical College of the Air Force Medical University,Xi′an 710032,China)

机构地区：[1]空军军医大学第一附属医院骨科,西安710032 [2]空军军医大学基础医学院神经生物学教研室暨脑科学协同创新中心,西安710032

出　　处：《中华手外科杂志》2020年第3期216-220,共5页Chinese Journal of Hand Surgery

基　　金：陕西省重点研发计划(2017ZDXM-SF-062)。

摘　　要：目的建立颈7根性撕脱诱发神经病理性痛的小鼠模型,并验证其可靠性。方法选用雌性C57小鼠36只,随机分为假手术组、撕脱伤组。撕脱伤组于前路行颈7根性撕脱术;假手术组仅暴露并分离颈7神经根。术前和术后,检测小鼠患侧前足痛行为、双侧前足肌力及Rota-rod实验。术后7 d,取颈7节段脊髓行免疫荧光染色,并进行统计学分析。结果与假手术组相比,撕脱伤组出现明显机械痛敏和冷痛敏(P<0.01),热痛敏、肌力和Rota-rod差异无统计学意义(P>0.05)。免疫荧光染色结果显示术后7 d,撕脱伤组脊髓背角GFAP及Iba1表达较假手术组显著升高(P<0.05)。结论此单纯颈7根性撕脱小鼠模型可显著诱发神经病理性痛,且对患肢运动功能无明显损伤,可作为研究臂丛神经撕脱伤诱发神经病理性痛的理想动物模型。Objective To establish a mouse model of neuropathic pain induced by C7 root avulsion and verify its reliability.Methods Thirty-six female C57 mice were randomly divided into sham operation group and brachial plexus avulsion group. In the avulsion group, the C7 root was avulsed through anterior approach;in the sham operation group, C7 root was exposed and separated. Before and after the operation, the pain behavior of the affected side of the forepaw, the muscle strength of the bilateral forepaws and the Rota-rod test were measured. At the 7th day after the operation, the segment of C7 spinal cord was havested to stain with immunofluorescence, and the data were analyzed statistically.Results Compared with the sham operation group, the avulsion group had significant mechanical and cold pain hypersensitivity (P<0.01), but there were no significant differences in thermal pain sensitivity, muscle strength and Rota-rod (P>0.05). The immunofluorescence staining results showed that the expression of GFAP and Iba1 in spinal dorsal horn of avulsion group was significantly higher than that of sham operation group 7 days after operation (P<0.05).Conclusion This simple C7 nerve avulsion mouse model can induce neuropathic pain and has no significant damage to the motor function of the affected limb. It can be used as an ideal animal model to study neuropathic pain induced by brachial plexus avulsion.

关 键 词：臂丛 动物实验 神经病理性痛 行为学检测 小鼠 

分 类 号：R-332[医药卫生] R688