Structural basis for gating mechanism of Pannexin 1 channel  

作　　者：Luqiu Mou Meng Ke Mengxiao Song Yuanyue Shan Qingjie Xiao Qingting Liu Jialu Li Ke Sun Lei Pu Li Guo Jia Geng Jianping Wu Dong Deng 

机构地区：[1]Division of Obstetrics,Key Laboratory of Birth Defects and Related Disease of Women and Children of MOE,State Key Laboratory of Biotherapy,West China Second Hospital,Sichuan University,Chengdu,610041 Sichuan,China [2]School of Life Sciences,Westlake University,Hangzhou,310024 Zhejiang,China [3]Institute of Biology,Westlake Institute for Advanced Study,Hangzhou,310024 Zhejiang,China [4]Key Laboratory of Structural Biology of Zhejiang Province,School of Life Sciences,Westlake University,Hangzhou,310024 Zhejiang,China [5]Department of Laboratory Medicine,State Key Laboratory of Biotherapy,West China Hospitai Sichuan University and Collaborative Innovation Center for Biotherapy,Chengdu,610041 Sichuan,China

出　　处：《Cell Research》2020年第5期452-454,共3页细胞研究（英文版）

基　　金：This work was supported by funds from National Key R&D Program of China(2016YFA0502700);Sichuan Youth Science and Technology Foundation(2017JQ0007).

摘　　要：Dear Editor,Pannexin 1(PANX1)plays extensive physiological roles across diverse fields of biology,including cell death,1,2 inflammation,3 cancer progression,4 and neurological disorders.5 As suggested by biochemical analysis,PANX1 forms an oligomeric channel for the facilitated diffusion of ions and large molecules across the plasma membrane upon activation.6 However,the underlying molecular gating mechanism of PANX1 and the structure of human PANX1 are still elusive.In the current study,we report the cryo-EM structures of full-length and carboxyl-terminal(CT)tail-cleaved human PANX1.Combined with single-channel electrophysiological study,we identified the key residues involved in the gating of PANX1.

关 键 词：INFLAMMATION MECHANISM CHANNEL 

分 类 号：Q25[生物学—细胞生物学]