CRISPR/Cas9技术在主要油料作物中的应用进展  

Advances in the application of CRISPR/Cas9 technology in main oil crops

作　　者：李麟芳 肖麓 LI Linfang;XIAO Lu(Institute of Spring Rapeseed,Qinghai Academy of Agriculture and Forestry Sciences/the Qinghai Research Branch of National Rapeseed Genetic Improvement Center/Scientific Observing and Experiment Station of Spring Rapeseed,Ministry of Agriculture and Rural Affairs/Key Laboratory of Qinghai Province for Spring Rapeseed Genetic Improvement/Spring Rapeseed Research and Development Center of Qinghai Province,Xining 810016,China)

机构地区：[1]青海大学农林科学院春油菜研究所国家油菜改良青海分中心农业农村部春油菜科学观测实验站青海省春油菜遗传改良重点实验室青海省春油菜研究开发中心,西宁810016

出　　处：《植物生理学报》2020年第3期373-381,共9页Plant Physiology Journal

基　　金：青海省春油菜遗传改良重点实验室项目(2017-ZJ-Y09);国家现代农业产业技术体系建设专项(CARS-12);春油菜区转基因油菜新品种培育子课题(2018ZX08020001-009)。

摘　　要：CRISPR/Cas9作为一种新兴的基因编辑技术,在作物育种中具有广阔的应用潜力,为油料作物如油菜(Brassica napus)、大豆(Glycine max)、花生(Arachis hypogaea)、芝麻(Sesamum indicum)等的基因功能和遗传改良研究提供了新的工具。当前,用该技术对油料作物的产量、品质相关性状基因定点编辑,获得了一系列的突变种质资源。此外,该技术也广泛应用于油料作物功能基因组研究中。本文对CRISPR/Cas9系统的转化方式、产生突变的检测方法及其在油料作物中的应用进行了总结,讨论了存在的相关问题,并对该技术在油料作物中的应用前景进行了展望,以期为相关应用研究提供参考。CRISPR/Cas9 system is an emerging gene-editing technology with great potential in crop breeding, providing a new tool for studying gene function and genetic improvement of oil crops, such as Brassica napus, Glycine max, Arachis hypogaea, Sesamum indicum, etc. At present, CRISPR/Cas9 system is mainly used in fixed-point editing of yield and quality-related traits in oil crops, and a series of mutant accessions were obtained. In addition, this system is widely used for functional genome research. This review summarizes the delivery patterns, mutation detection and application of CRISPR/Cas9 system in oil crops, discusses the existing problems, and prospects for future applications in this field, providing references for relative studies.

关 键 词：CRISPR/Cas9 油料作物 转化方式 突变检测 应用进展 

分 类 号：S565[农业科学—作物学]