Arabidopsis PEAPODs function with LIKE HETEROCHROMATIN PROTEIN1 to regulate lateral organ growth  

作　　者：Ying Zhu Xiao Luo Xuxin Liu Wenjuan Wu Xiaofeng Cui Yuehui He Jirong Huang 

机构地区：[1]National Key Laboratory of Plant Molecular Genetics,Shanghai Institute of Plant Physiology and Ecology,Chinese Academy of Sciences,Shanghai 200032,China [2]National Key Laboratory of Plant Molecular Genetics,Shanghai Center for Plant Stress Biology,CAS Center for Excellence in Molecular Plant Sciences,Chinese Academy of Sciences,Shanghai 201602,China [3]Shanghai Key Laboratory of Plant Molecular Sciences,College of Life Sciences,Shanghai Normal University,Shanghai 200234,China [4]Xinjiang Agricultural Vocational Technical College

出　　处：《Journal of Integrative Plant Biology》2020年第6期812-831,共20页植物学报（英文版）

基　　金：supported by grants from National Science Foundation of China(31771547);Chinese National Special Grant for Transgenic Crops(2016ZX08009003-005)。

摘　　要：In higher plants,lateral organs are usually of determinate growth.It remains largely elusive how the determinate growth is achieved and maintained.Previous reports have shown that Arabidopsis PEAPOD(PPD)proteins suppress proliferation of dispersed meristematic cells partly through a TOPLESS corepressor complex.Here,we identified a new PPD-interacting partner,LIKE HETEROCHROMATIN PROTEIN1(LHP1),using the yeast two-hybrid system,and their interaction is mediated by the chromo shadow domain and the Jas domain in LHP1 and PPD2,respectively.Our genetic data demonstrate that the phenotype of ppd2 lhp1 is more similar to lhp1 than to ppd2,indicating epistasis of lhp1 to ppd2.Microarray analysis reveals that PPD2 and LHP1 can regulate expression of a common set of genes directly or indirectly.Consistently,chromatin immunoprecipitation results confirm that PPD2 and LHP1 are coenriched at the promoter region of their targets such as D3-TYPE CYCLINS and HIGH MOBILITY GROUP A,which are upregulated in ppd2,lhp1 and ppd2 lhp1 mutants,and that PPDs mediate repressive histone 3 lysine-27 trimethylation at these loci.Taken together,our data provide evidence that PPD and LHP1 form a corepressor complex that regulates lateral organ growth.

关 键 词：ARABIDOPSIS FUNCTION ORGANS 

分 类 号：Q943.2[生物学—植物学]