18α-甘草次酸对IHH-4细胞FTO表达及增殖、迁移的影响  

The influences of 18α-Glycyrrhetinic acid on the expression of FTO and proliferation and migration in IHH-4

作　　者：李倩[1] 罗昭锋 郭晓磊[1] 刘晓丽 张自森[2] Li Qian;Luo Shaofeng;Guo Xiaolei;Liu Xiaoli;Zhang Zisen(Department of Thyroid Surgery,the Fifth Affiliated Hospital of Zhengzhou University,Henan Zhengzhou 450052,China;Department of Oncology,the Fifth Affiliated Hospital of Zhengzhou University,Henan Zhengzhou 450052,China)

机构地区：[1]郑州大学第五附属医院甲状腺外科,河南郑州450052 [2]郑州大学第五附属医院肿瘤科,河南郑州450052

出　　处：《现代肿瘤医学》2020年第13期2199-2203,共5页Journal of Modern Oncology

基　　金：河南省医学科技攻关项目(编号:201701015)。

摘　　要：目的:评价肥胖相关基因(fat mass and obesity associated gene,FTO)蛋白在人甲状腺乳头状癌IHH-4细胞中的表达、生物学作用及18α-甘草次酸(18α-Glycyrrhetinic acid,AGA)对该细胞FTO表达的影响。方法:体外培养IHH-4细胞,取对数生长期细胞进行实验;采用MTT和Transwell迁移实验评价不同浓度的AGA(80、160、320μmol/L)对IHH-4细胞增殖和迁移能力的影响;通过RNA干扰(RNA interference,RNAi)技术沉默肥胖相关基因(fat mass and obesity associated gene,FTO)的表达;采用Western blot法评价AGA处理前后FTO、周期蛋白依赖性激酶4(cyclin-dependent kinase 4,CDK4)和Cofilin-1的表达。结果:不同浓度的AGA均能显著抑制IHH-4细胞的增殖及迁移,差异具有统计学意义(P<0.05);IHH-4细胞高表达FTO、CDK4和Cofilin-1;RNAi下调IHH-4细胞FTO后导致CDK4和Cofilin-1表达水平显著降低(P<0.05),IHH-4细胞的增殖及迁移能力亦显著下降;AGA处理显著减少了IHH-4细胞中FTO、CDK4和Cofilin-1的表达水平(P<0.05);经RNAi处理48 h的IHH-4细胞再加入AGA无法进一步降低FTO、CDK4和Cofilin-1的表达水平及增殖、迁移能力(P>0.05)。结论:FTO在促进人甲状腺乳头状癌细胞的增殖及迁移中具有重要作用;AGA通过控制FTO的表达抑制IHH-4细胞的增殖及迁移。Objective:To assess the expression of fat mass and obesity associated gene(FTO),its biological function and the effects of 18α-Glycyrrhetinic acid(AGA)on FTO in IHH-4 cells.Methods:IHH-4 cells were cultured in vitro and these cells were used in experiments when they were in logarithmic phase.MTT and Transwell tests were used to examine the influences of AGA(80,160 and 320μmol/L)on the proliferation and migration of IHH-4 cells,respectively.RNA interference(RNAi)method was used to downregulate the expression of FTO.The levels of FTO,cyclin-dependent kinase 4(CDK4)and Cofilin-1 were evaluated by Western blot.Results:Different doses of AGA could significantly suppress the proliferation and migration of IHH-4 cells.IHH-4 cells expressed high levels of FTO,CDK4 and Cofilin-1.Knockdown of FTO led to significant dowregualtion of CDK4 and Cofilin-1 and suppression of proliferation and migration of IHH-4 cells.AGA treatment(320μmol/L)dramatically reduced the expression of FTO,CDK4 and Cofilin-1.However,AGA treatment after knockdown of FTO could not further reduce the levels of FTO,CDK4 and Cofilin-1 in IHH-4 cells,and the proliferation and migration of IHH-4 cells could not be reduced compared to those in RNAi group.Conclusion:FTO plays a vital role in the proliferation and migration of IHH-4 cells.AGA can inhibit the proliferation and migration of IHH-4 cells via downregualtion of FTO.

关 键 词：IHH-4细胞 肥胖相关基因 增殖 迁移 18α-甘草次酸 

分 类 号：R736.1[医药卫生—肿瘤]