STAT3基因沉默对胰腺癌细胞放射敏感性的影响及其机制  

Effect of STAT3 gene silencing on radiosensitivity of pancreatic cancer cells and its mechanism

作　　者：杨麒麟[1] 王国红 杨霞[1] Yang Qilin;Wang Guohong;Yang Xia(Oncology Department,Ziyang People's Hospital,Sichuan Ziyang 641300,China)

机构地区：[1]资阳市人民医院肿瘤科,四川资阳641300

出　　处：《现代肿瘤医学》2020年第13期2203-2209,共7页Journal of Modern Oncology

基　　金：四川省卫生卫科研项目(编号:110575)。

摘　　要：目的:探讨信号转导和转录激活因子3(STAT3)基因沉默对人胰腺癌细胞AsPC-1和PANC-1放射敏感性的影响及其机制。方法:体外培养人胰腺癌细胞AsPC-1和PANC-1,通过脂质体转染STAT3 shRNA作为实验组(shSTAT3组),设置阴性对照(shNC组)和空白对照组(NC组)。采用qRT-PCR检测转染后各组AsPC-1和PANC-1细胞中STAT3 mRNA表达水平,Western blot检测细胞中STAT3蛋白表达水平。MTT实验检测各组细胞增殖能力,流式细胞仪检测各组细胞电子射线照射后的凋亡情况,qRT-PCR和Western blot分别检测各组细胞电子射线照射后的Bax、Bcl-2和Caspase-3 mRNA和蛋白的表达情况。结果:shSTAT3组AsPC-1和PANC-1细胞中STAT3 mRNA和蛋白表达水平比NC组和shNC组明显降低(P<0.05)。接受电子射线照射后,shSTAT3组AsPC-1和PANC-1细胞增殖率显著低于shNC组(P<0.05),其细胞凋亡率较shNC组明显升高(P<0.05)。接受电子射线照射后,转染STAT3 shRNA后能够明显上调细胞中Bax和Caspase-3的表达和下调Bcl-2的表达(P<0.05)。结论:沉默STAT3基因能够通过上调Bax和Caspase-3的表达和下调Bcl-2的表达促进细胞凋亡,增强胰腺癌细胞的放射敏感性。Objective:To investigate the effect of signal transduction and activator of transcription 3(STAT3)gene silencing on the radiosensitivity of human pancreatic cancer cell AsPC-1,PANC-1 and its mechanism.Methods:Human pancreatic cancer cell AsPC-1,PANC-1 was cultured in vitro,transfected with STAT3 shRNA by liposome as experimental group(shSTAT3 group),negative control(shNC group)and blank control group(NC group)were set.The expression of STAT3 mRNA in AsPC-1 and PANC-1 cells was detected by qRT-PCR.The expression of STAT3 protein was detected by Western blot.The plate cloning assay was used to detect the radiosensitivity of each group.Flow cytometry was used to detect apoptosis after electron beam irradiation in each group.qRT-PCR and Western blot were used to detect the expression of Bax,Bcl-2 and Caspase-3 mRNA and protein after electron beam irradiation in each group.Results:The expression levels of STAT3 mRNA and protein in AsPC-1 and PANC-1 cells of shSTAT3 group were significantly lower than those in NC group and shNC group(P<0.05).After electron beam irradiation,the survival fraction of shSTAT3 group AsPC-1 and PANC-1 cells was significantly lower than that of the shNC group(P<0.05).The apoptotic rate was significantly higher than that of the shNC group(P<0.05).After receiving electron beam irradiation,transfection of STAT3 shRNA can significantly up-regulate the expression of Bax and Caspase-3 and down-regulate the expression of Bcl-2 in cells(P<0.05).Conclusion:Silencing STAT3 can promote apoptosis and up-regulate the sensitivity of pancreatic cancer cells by up-regulating Bax expression and down-regulating Bcl-2 expression.

关 键 词：STAT3基因 胰腺癌细胞AsPC-1 放射敏感性 BAX Bcl-2 

分 类 号：R735.9[医药卫生—肿瘤]