3D打印个体化钛网的机械力学性能及生物相容性分析  

Mechanical properties of 3D-printed titanium mesh and its biocompatibility in vitro

作　　者：张耀升 张锴 陈欣慰[3] 牟海彰 丁旺旺 秦明礼 张善勇[3] 龚勤林[2] 陈刚 徐伟峰[3] 虞科恩 姜艳梅 ZHANG Yao-sheng;ZHANG Kai;CHEN Xin-wei;MU Hai-zhang;Ding Wang-wang;Qin Ming-li;ZHANG Shan-yong;GONG Qin-lin;Chen Gang;XU Wei-feng;YU Ke-en;JIANG Yan-mei(College of Stomatology,Weifang Medical University.Weifang 261053,Shandong Province;Sichuan University.Chengdu 610065,Sichuan Province;Department of Oral Surgery,Shanghai Ninth People's Hospital,College of Stomatology,Shanghai Jiao Tong University School of Medicine,Shanghai Key Laboratory of Stomatology.Shanghai 200011;University of Science and Technology Beijing.Beijing 100083,China)

机构地区：[1]潍坊医学院口腔医学院,山东潍坊261053 [2]四川大学,四川成都610065 [3]上海交通大学医学院附属第九人民医院·口腔医学院口腔外科,上海口腔医学重点实验室,上海200011 [4]北京科技大学新材料技术研究院,北京100083

出　　处：《上海口腔医学》2020年第3期250-256,共7页Shanghai Journal of Stomatology

基　　金：2016年浦东新区卫计委联合攻关项目(PW2016D-1);四川大学大学生创新创业训练计划项目(T2020109750);上海市卫生和计划生育委员会面上项目(M20170232)。

摘　　要：目的:比较3D打印钛网与成品钛网的机械力学性能差异,并评价3D打印钛网对细胞生长及分化的影响.方法:采用激光打印技术制备个体化3D打印钛网,用静态拉伸压缩载荷实验检测机械力学性能,评估机械力学性能.选择4周龄雄性SD大鼠骨髓间充质干细胞,与不同孔径大小的3D打印钛网共培养.采用CCK-8法检测各组细胞增殖情况;对各组细胞进行成骨诱导分化后,检测碱性磷酸酶(ALP)活性改变,运用实时荧光定量PCR检测相关成骨基因的表达;通过扫描电镜及活/死细胞染色,观察细胞在3D打印钛网上的黏附和生长情况.采用SPSS22.0软件包对数据进行统计学分析.结果:3D打印钛网的拉伸压缩负载实验结果显示其具有优异的力学性能;相比对照组,不同孔径钛网对细胞增殖的影响不大,细胞在钛网上显现出良好的黏附和生长状态.3D打印钛网对成骨分化具有一定的促进作用.结论:3D打印钛网的机械力学性能优异,且具有良好的生物相容性.PURPOSE:To compare the mechanical properties of 3D-printed titanium meshes and pre-shaped titanium meshes,and to evaluate the effects of 3D-printed titanium meshes on cell proliferation and differentiation.METHODS:3D-printed titanium meshes were produced and prepared with laser printing machine.The mechanical properties were analyzed by static tension and compression load test.Bone marrow mesenchymal stem cells(BMSCs)were extracted from 4-week-old male SD rats.BMSCs were co-cultured with 3D-printed titanium meshes of different apertures.Cell counting kit-8(CCK-8)assay was used to detect cell proliferation.Alkaline phosphatase(ALP)activity assay was used to test ALP activity.The expression of related osteogenic genes was tested by real-time PCR.The adhesion and growth of BMSCs were investigated by scanning electron microscopy(SEM)and living/dead cell staining.SPSS 22.0 software package was used for statistical analysis of the results.RESULTS:The results of 3D-printing Ti-meshes tension and compression loading experiment were excellent.The 3D-printing Ti-meshes showed no inhibitory effects on cell proliferation,survival and adhesion,but had a positive effect on osteogenesis of BMSCs.CONCLUSIONS:The mechanical properties of 3D-printed Ti-meshes are excellent.The 3D-printed Ti-meshes have good biocompatibility.

关 键 词：3D打印钛网 机械力学性能 生物相容性 

分 类 号：R782.1[医药卫生—口腔医学]