牛磺酸对宫内生长受限孕鼠生长轴激素与基因的影响  

Effects of taurine on growth axis hormones and genes in pregnant rats with intrauterine growth restriction

作　　者：李平丽 吴骁 杨建成[1] LI Pingli;WU Xiao;YANG Jiancheng(College of Veterinary and Animal Science,Shenyang Agricuhural University,Shenyang Liaoning 110866,China;Liaoning He’s Medical College,Shenyang 110163)

机构地区：[1]沈阳农业大学畜牧兽医学院,沈阳110866 [2]辽宁何氏医学院动物实验中心,沈阳110163

出　　处：《中国实验动物学报》2020年第3期345-350,共6页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家自然科学基金项目(31572481,31872441)。

摘　　要：目的观察牛磺酸对宫内生长受限孕鼠血液激素与IGF基因表达的影响。方法取健康SD系大鼠雌雄2∶1合笼饲养,按妊娠顺序随机抽取孕鼠分为正常饲料组(C)、低蛋白饲料组(M)、低蛋白饲料+牛磺酸组(MT),正常饲料+牛磺酸组(T)。每组孕鼠各10只,M、MT组孕期采取低蛋白饲料喂养造模,孕第12天,C和M组孕鼠灌胃饮用水,MT和T组灌胃牛磺酸300 mg/(kg·d)。每周检测孕鼠体重增长情况,孕21 d取样,记录胎鼠体重、数量、畸胎数;Elisa方法检测孕鼠血清生长激素(GH)、胰岛素生长因子1(IGF-1)、胰岛素(INS)、甲状腺素(T3和T4)水平,荧光定量PCR方法检测孕鼠肝生长激素受体(GHR)、胰岛素生长因子1(IGF-1)、胰岛素生长因子结合蛋白1(IGFBP-1)和胰岛素生长因子结合蛋白3(IGFBP-3)的mRNA表达量。结果添加牛磺酸组胎鼠体重、数量和畸胎明显改善,孕鼠体重增加显着(P<0.05),血液INS和T4增加显着(P<0.05),肝IGF-1、IGFBP-3 mRNA表达升高显着(P<0.05),IGFBP-1 mRNA显着降低(P<0.01)。结论添加牛磺酸能够改善低蛋白孕鼠甲状腺功能、GH/IGF-1轴紊乱,这可能在缓解胎儿子宫生长受限中具有重要的作用。Objective To observe the effects of taurine on blood hormones and hepatic insulin growth factor(IGF)gene expression in intrauterine growth-restricted pregnant rats.Methods Healthy SD female and male rats were housed 1∶2 in cages and pregnant rats were randomly selected into the blank control group(C),model group(M),model+taurine group(MT),and taurine group(T).In the M and MT groups,10 pregnant rats were fed a low-protein diet.Day 12 of pregnancy,C,and M were provided drinking water,and MT and T groups received taurine 300 mg/(kg·d).Weekly monitoring of weight gain in pregnant rats.At day 21 of gestation,baseline characteristics of fetuses were determined.Serum growth hormone(GH),insulin growth factor 1(IGF-1),insulin(INS),and thyroxine(T3 and T4)levels were detected by ELISA.Fluorescent quantitative PCR was used to detect the hepatic expression levels of growth hormone receptor(GHR),insulin growth factoR-1(IGF-1),insulin growth factor binding protein-1(IGFBP-1)and insulin growth factor binding protein-3(IGFP-3)mRNA in pregnant rats.Results Taurine markedly improved the baseline characteristics,compared with the M group.Pregnant rats in the MT group had significantly increased(P<0.05)weights,blood INS and T4 levels,and liver IGF-1 and IGFBP-3 mRNA expression,whereas IGFBP-1 mRNA expression was significantly reduced(P<0.01).Conclusions The addition of taurine can improve the thyroid function and GH/IGF-1 axis disorder in lowprotein diet pregnant rats,and may play an important role in alleviating fetal growth restriction.

关 键 词：牛磺酸 生长受限孕鼠 胰岛素样生长因子1 

分 类 号：Q95-33[生物学—动物学]