灵芝多糖联合二甲双胍对2型糖尿病大鼠炎症因子及胸主动脉血管细胞黏附分子-1表达的影响  

Effect of ganoderma lucidum polysaccharide combining with metformin on the expression of inflammatory factors and VCAM-1 in the thoracic aorta of type 2 diabetic rats

作　　者：乔进[1] 施凯[1] 陈惠[2] 郑惠华[2] 徐济良[3] QIAO Jin;SHI Kai;CHEN Hui;ZHENG Hui-hua;XU Ji-liang(Department of Pharmacy,the Third People’s Hospital of Nantong,Nantong Jiangsu 226006;Jiangsu Anhui Biological Technology Co.Ltd,Nantong Jiangsu 226000;School of Medicine,Nantong University,Nantong Jiangsu 226006)

机构地区：[1]南通市第三人民医院药学部,江苏南通226006 [2]江苏安惠生物科技有限公司,江苏南通226000 [3]江苏省南通大学医学院,江苏南通226006

出　　处：《中南药学》2020年第6期978-981,共4页Central South Pharmacy

基　　金：“十一五”国家科技支撑计划子课题项目(No.2006DAI06A20-02);南通市临床医学中心项目(No.HS2016002)。

摘　　要：目的探讨灵芝多糖联合二甲双胍对2型糖尿病大鼠炎症因子及胸主动脉血管黏附分子-1(VCAM-1)表达的影响。方法 SD大鼠高脂饮食4周后腹腔注射链脲佐菌素(STZ)30 mg·kg^-1建立2型糖尿病(T2DM)大鼠模型,随机分成糖尿病组、灵芝多糖组(600 mg·kg^-1)、二甲双胍组(600mg·kg^-1)及联合用药组(灵芝多糖300 mg·kg^-1+二甲双胍300 mg·kg^-1),另设正常对照组,每组10只大鼠。灌胃给药,每日1次,正常对照组和糖尿病组给予生理盐水。12周末颈动脉取血,测定血清低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)、白介素-6(IL-6)、肿瘤坏死因子(TNF-α)及C反应蛋白(CRP),免疫组化法检测主动脉VCAM-1的表达。结果联合用药组能显著降低血清LDL-C、IL-6、TNF-α、CRP及升高HDL-C水平,并对主动脉VCAM-1表达有明显抑制作用。结论灵芝多糖联合二甲双胍对预防糖尿病大鼠动脉粥样硬化形成具有协同作用,其机制可能与调节血脂、抗炎作用及抑制VCAM-1的表达有关。Objective To determine the effect of ganoderma lucidum polysaccharides combining with metformin on the expression of inflammatory factors and vascular cell adhesion molecule-1 VCAM-1 in the thoracic aorta of type 2 diabetic rats.Methods SD rats were fed with high fat diet for 4 weeks,and then was injected to replicate type 2 diabetic models with STZ(30 mg·kg^-1).The rats were randomly divided into a diabetes group,a ganoderma lucidum polysaccharides group(600 mg·kg^-1),a metformin group(600 mg·kg^-1),a combination group(ganoderma lucidum polysaccharides 300 mg·kg^-1+metformin group 300 mg·kg^-1),and a normal control group,10 rats in each group with intragastric administration,once daily.The normal control group and diabetes group were fed with normal saline.After the 12 weeks treatment,blood samples were drawn from the carotid artery of rats,the levels of LDL-C,HDL-C,IL-6,TNF-αand CRP in the serum were measured with biochemical method.Immunohistochemical method was used to detect VCAM-1 protein expression in the thoracic aorta.Results Combined use of ganoderma lucidum polysaccharides and metformin decreased the levels of LDL-C,IL-6,TNF-αand CRP remarkably,while increased the level of HDL-C significantly.The expression of VCAM-1 in the atherosclerotic aorta decreased remarkably in the combination group.Conclusion Ganoderma lucidum polysaccharides combining with metformin synergistically prevent against atherosclerosis in type 2 diabetic rats.The mechanism may be related to regulating the plasma lipid profile,inhibiting inflammation and down regulating the expression of VCAM-1 in the thoracic aorta.

关 键 词：灵芝多糖 二甲双胍 糖尿病大鼠 动脉粥样硬化 血管细胞黏附分子-1 

分 类 号：R285[医药卫生—中药学]