黄瓜叶色黄化突变基因yl-2的鉴定  

Identification of the Mutant Gene yl-2 Conferring the Yellow Leaf Trait in Cucumber

作　　者：李洋洋 薛冰 周倩 王博文 陈宸[5] 陈惠明[6] 杨学勇[2] 张忠华[2] 李征[1] 王深浩 LI Yang-yang;XUE Bing;ZHOU Qian;WANG Bo-wen;CHEN Chen;CHEN Hui-ming;YANG Xue-yong;ZHANG Zhong-hua;LI Zheng;WANG Shen-hao(College of Horticulture,Northwest A&F University,Yangling 712100,Shaanxi,China;Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China;School of Food and Biological Engineering,Hefei University of Technology,Hefei 230601,Anhui,China;China Agricultural Genome Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen 518124,Guangdong,China;College of Bioscience and Biotechnology,Hunan Agricultural University,Changsha 410125,Hunan,China;Hunan Vegetable Research Institute,Changsha 410125,Hunan,China)

机构地区：[1]西北农林科技大学园艺学院,陕西杨陵712100 [2]中国农业科学院蔬菜花卉研究所,北京100081 [3]合肥工业大学食品与生物工程学院,安徽合肥230601 [4]中国农业科学院深圳农业基因组研究所,广东深圳518124 [5]湖南农业大学生物科学技术学院,湖南长沙410125 [6]湖南省蔬菜研究所,湖南长沙410125

出　　处：《中国蔬菜》2020年第7期30-37,共8页China Vegetables

基　　金：国家自然科学基金项目(31701914);中国博士后科学基金项目(2017M613227,2018T111109)。

摘　　要：叶绿素的含量与叶片光合作用效率以及作物产量潜力密切相关,是作物的一个重要生理指标。通过对黄瓜株系HN3种子进行甲磺酸乙酯(EMS)诱变,筛选得到叶色黄化的突变体yl-2。以该叶色黄化突变体yl-2和野生型黄瓜HN3为亲本,构建了BC1F2分离群体,遗传分析表明该突变体为隐性单基因控制。通过DNA混池测序,运用MutMap的方法寻找突变基因,得到4个符合要求的SNP位点,这4个SNP位点分布在黄瓜6号染色体上13~19 Mb的物理区间内。在这4个SNP中,仅有1个SNP即SNP17451330位于基因(Csa6G385090)上,且为异义突变,其余3个SNP均位于基因间。生物信息分析发现基因Csa6G385090编码叶绿素a加氧酶CAO,SNP17451330导致的氨基酸突变位于CAO蛋白的保守结构域。根据异义突变SNP17451330设计的dCAPS标记与群体植株的叶色表型共分离,预示着基因Csa6G385090很可能是叶色黄化突变体yl-2的候选基因。Chlorophyll content is closely related to leaf photosynthesis efficiency and crop yield potential,and it is also an important physiological index of crops.Through conducting EMS mutagenesis on seeds of cucumber line HN3,the mutant yl-2 with yellow leaf was screened and obtained.Taking the yellow leaf mutant yl-2 and wild-type cucumber HN3 as parents,this study constructed a BC1F2 seperation population.Genetic analysis showed that this mutant was controlled by a recessive single gene.Through DNA pooling sequencing,we used MutMap strategy to find mutant gene,and obtained 4 satisfactory SNP loci.These 4 SNP loci were distributed in the 13-19 Mb physical interval on cucumber chromosome 6.Only one SNP i.e.SNP17451330 among these 4 SNP was a missense mutation,located on the gene(Csa6G385090),and the other 3 were all located on intergenic variants.By biological information analysis we found that gene Csa6G385090 encoded chlorophyll a oxygenase CAO,and amino acid mutation caused by SNP17451330 was located in the conserved domain of CAO protein.The dCAPS marker designed according to SNP17451330 was co-separated with the leaf color phenotype of the population plants,indicating that Csa6G385090 was most likely to be the candidate gene for the yellow leaf mutant yl-2.

关 键 词：黄瓜 叶色黄化突变体 MutMap 

分 类 号：S642.2[农业科学—蔬菜学]