血糖敏感型水凝胶治疗糖尿病大鼠牙周炎的实验研究  

A Study on Glucose-sensitive Hydrogel in the Treatment of Periodontitis in Diabetic Rats

作　　者：刘君瑜 肖宇[2] 王翔宇[1] 刘艳 李玉芳 柳海凤 陈宇[2] 包崇云[2] LIU Junyu;XIAO Yu;WANG Xiangyu;LIU Yan;LI Yufang;LIU Haifeng;CHEN Yu;BAO Chongyun(Shanxi Medical University School and Hospital of Stomatology,Shanxi Province Key Laboratory of Oral Diseases Prevention and New Materials,Clinical Medical Research Center of Oral Diseases of Shanxi Province (Cultivation), Taiyuan 030002, China;State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Disease, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China;Department of Implantology, School of Stomatology, The Fourth Military Medical University, Xi’an 710032, China)

机构地区：[1]山西医科大学口腔医学院·口腔医院,口腔疾病防治与新材料山西省重点实验室,山西省口腔疾病临床医学研究中心(培育),山西太原030002 [2]口腔疾病研究国家重点实验室,国家口腔疾病临床医学研究中心,四川大学华西口腔医学院,四川成都610041 [3]第四军医大学口腔医院口腔种植科,陕西西安710032

出　　处：《口腔医学研究》2020年第6期567-571,共5页Journal of Oral Science Research

基　　金：国家自然科学基金(编号:81701027);山西省高等学校科技创新项目(编号:2019L0432);山西省高等学校教学改革创新项目(编号:J2019076);山西医科大学青年基金(编号:02201521);山西医科大学口腔医院科研项目(编号:KY201806)。

摘　　要：目的:制备血糖敏感型药物控释水凝胶用于糖尿病大鼠实验性牙周炎的治疗。方法:以壳聚糖作为基质,应用光交联法来制备水凝胶,并将葡萄糖氧化酶固定于该水凝胶上制备血糖敏感型水凝胶。用稳定性测试和细胞粘附实验对其进行性能表征,并且将载甲硝唑水凝胶浸泡在动态变化的葡萄糖溶液中进行药物释放实验,测试其响应血糖的能力,最后建立糖尿病牙周炎大鼠模型,将水凝胶置入实验性牙周袋中,观察其治疗效果。结果:本实验运用光交联法制备的水凝胶机械性能稳定,利于成骨细胞MC3T3-E1粘附,该材料表面固定葡萄糖氧化酶后,甲硝唑释放量随着动态变化的血糖中而改变。体内实验结果显示使用载药水凝胶后,大鼠牙周组织肿胀、出血减轻,愈合情况良好。结论:本实验制备的血糖敏感型壳聚糖水凝胶可以为糖尿病牙周炎的辅助药物治疗提供一种新的思路。Objective:To prepare glucose-sensitive controlled-release hydrogel for the treatment of periodontitis in diabetic rats models.Methods:Chitosan was used as matrix material to prepare hydrogel.Glucose oxidase was immobilized on the hydrogel to prepare glucose sensitive hydrogel,which was characterized by stability test and cell adhesion test.Metronidazole-loaded hydrogel was immersed in dynamic changing concentration of glucose solution.Drug release test was used to test its ability to respond to blood glucose.Finally,Hydrogel was put into the experimental periodontal pocket of diabetic rats to observe its therapeutic effects.Results:The photo-crosslinked hydrogel had stable mechanical properties and facilitated the adherence of osteoblasts MC3T3-E1.After glucose oxidase was immobilized on the surface of the hydrogel,metronidazole release was changed with different concentration of glucose.In the in vivo study,the swelling and bleeding of periodontal tissue were alleviated.Conclusion:A novel glucose sensitive hydrogel drug delivery system is prepared in this study,which provides a promising method for the treatment of diabetic periodontitis.

关 键 词：血糖敏感 壳聚糖 牙周炎 药物控释 

分 类 号：R78[医药卫生—口腔医学]