Streptomyces sp.FIM-16-06活性物质的分离纯化与结构鉴定  

Isolation,Purification and Structural Identification of a Bioactive Metabolite from Fermentation Broth of Streptomyces sp.FIM-16-06

作　　者：王德森[1,2] 张祝兰 程贤[1] 杨煌建 邱观荣[1] 连云阳[1] WANG De-sen;ZHANG Zhu-lan;CHENG Xian;YANG Huang-jian;QIU Guan-rong;LIAN Yun-yang(Fujian Provincial Key Laboratory of Screening for Novel Microbial Products,Fujian Institute of Microbiology,Fuzhou 350007,China;Fujian Kerui Pharmaceutical Co.,Lid.,Fuzhou 350007,China)

机构地区：[1]福建省微生物研究所福建省新药(微生物)筛选重点实验室,福建福州350007 [2]福建科瑞药业有限公司,福建福州350007

出　　处：《药物生物技术》2020年第2期102-106,共5页Pharmaceutical Biotechnology

基　　金：福建省属公益类研究所基本科研专项(No.2019R1005-8);福州市科技计划项目(No.2018-G-48);福清市科技计划项目(No.融科2018(6))。

摘　　要：在筛选微生物代谢生物活性物质过程中,获得一株抑制免疫活性强的次级代谢产物产生菌Streptomyces sp.FIM-16-06,采用柱层析与结晶技术对Streptomyces sp.FIM-16-06发酵活性产物进行分离和纯化,得到一个大环内酯类化合物AS1606。经理化性质研究和波谱分析,确定由Streptomyces sp.FIM-16-06产生的次级代谢产物AS1606的化学结构与他克莫司相同。Streptomyces sp.FIM-16-06 with high immunosuppressive activity was isolated in the course of screening for bioactive microbial products.The target compound AS1606 was isolated and purified by chromatography and crystallization methods.Based on the physico-chemical properties and spectroscopic analysis of UV,MS,IR,1 HNRM,13 CNMR,DEPT,HMBC and HMQC,the active component AS1606 was structurally elucidated as the known immunosuppressant tacrolimus.

关 键 词：链霉菌 他克莫司 分离纯化 结构鉴定 质谱裂解规律 质谱 

分 类 号：R978.15[医药卫生—药品]