高效液相色谱法测定鱼粉中色氨酸及不确定度评定  

Uncertainty evaluation of tryptophan in fishmeal by high performance liquid chromatography method

作　　者：李兰[1] 贾铮[1] 周剑[1] 王敏[1] 樊霞[1] LI Lan;JIA Zheng;ZHOU Jian;WANG Min;FAN Xia(Key Laboratory of Agricultural Product Quality and Safety Research of Ministry of Agriculture,Institute of Quality Standard and Testing Technology for Agro-Products,Chinese Academy of Agricultural Sciences,Beijing 100081,China)

机构地区：[1]中国农业科学院农业质量标准与检测技术研究所,农业农村部农产品质量安全重点实验室,北京100081

出　　处：《食品安全质量检测学报》2020年第9期2714-2721,共8页Journal of Food Safety and Quality

基　　金：“十三五”国家重点研发计划(2016YFF0201105);中国农业科学院创新工程。

摘　　要：目的探讨利用高效液相色谱仪(high performance liquid chromatography,HPLC)测定饲料中色氨酸含量的方法,并对动物性饲料原料鱼粉中色氨酸含量检测过程的不确定度进行计算和评定。方法采用高效液相色谱仪,通过优化前处理过程和色谱条件,建立饲料中色氨酸含量的高效液相色谱测定方法,并针对鱼粉进行实际样品测定。依据测量不确定度评定和表示的要求,通过获取的数据对高效液相色谱法测定鱼粉中色氨酸过程的不确定度进行评定。结果选择的智利鱼粉中色氨酸含量为0.64%,测量结果的不确定度为0.01%;色氨酸检测过程中,样品的水解、试液的转移稀释、标准物质的纯度以及制备过程、液相色谱测定的重复性是测量误差的主要来源。结论本研究建立的液相色谱法可用于饲料中色氨酸含量的测定,同时构建的鱼粉中色氨酸含量的不确定度评定方法为检测过程的质量控制提供了参考。Objective To investigate the method for the determination of tryptophan content in feed by high performance liquid chromatography(HPLC),and calculate and evaluate the uncertainty of the determination process of tryptophan in the fishmeal of animal feedstuff.Methods The HPLC method was used in this study.The conditions for pre-possessing and chromatography were optimized in order to establish a method for measuring the concentration of tryptophan in fish meal.The uncertainty during the measurement of tryptophan was evaluated based on the requirements of evaluation and expression of uncertainty in measurement.Results The results showed that the tryptophan concentration in the Chilean fish meal and the uncertainty of tryptophan was 0.64%and 0.01%,respectively.Hydrolysis of samples,transfer dilution of test solution,purity of reference materials,preparation process and repeatability of liquid chromatography were the main sources of measurement error of the uncertainty.Conclusion The method established in this study can be used to evaluate the concentration of tryptophan in feed.The uncertainty evaluation method of the determination of tryptophan in fish meal provides a reference for the quality control of the detection process.

关 键 词：高效液相色谱 饲料 鱼粉 色氨酸 不确定度 

分 类 号：O657.72[理学—分析化学] S963.321[理学—化学]