高效液相色谱法同时测定磺胺喹噁啉二甲氧苄啶预混剂中两种主要成分含量  

Simultaneous determination of 2 main components in sulfaquinoxoline dimethoprim premix by high performance liquid chromatography

作　　者：谢丽丽 陈锡龙[1] 王庆红[1] 孙真峥 赵贵[1] XIE Li-Li;CHEN Xi-Long;WANG Qing-Hong;SUN Zhen-Zheng;ZHAO Gui(Guizhou Provincial Supervisory Institute of Veterinary Drug and Feeds,Guiyang 550003,China)

机构地区：[1]贵州省兽药饲料监察所,贵阳550003

出　　处：《食品安全质量检测学报》2020年第9期2842-2847,共6页Journal of Food Safety and Quality

基　　金：贵州省科技计划项目(黔科合支撑〔2019〕2318号);贵州省科技计划项目(黔科合支撑〔2019〕2320号)。

摘　　要：目的建立高效液相色谱法(high performance liquid chromatography,HPLC)同时测定磺胺喹噁啉二甲氧苄啶预混剂中磺胺喹噁啉和二甲氧苄啶含量的分析方法。方法采用Waters Symmetry C18分析柱(250 mm×4.6 mm,5μm),以0.1%磷酸-乙腈(75:25)为流动相,流速为1.0 mL/min,检测波长为240 nm,采用外标法进行定量。结果在此色谱条件下,2种组分分离度良好,分别在5.0~100μg/mL、1.0~20μg/mL范围内线性关系良好(r^2=0.99998);磺胺喹噁啉按0.2 mg/mg添加,平均回收率为97.86%(RSD=0.8%),二甲氧苄啶按0.04 mg/mg添加,平均回收率95.14%(RSD=1.9%)。结论该方法简便、快速、准确,重复性良好,可用于磺胺喹噁啉二甲氧苄啶预混剂中磺胺喹噁啉和二甲氧苄啶的同时测定。Objective To establish a method for the simultaneous determination of sulfaquinoxaline and dimethoxidine in sulfaquinoxaline dimethoxidine premix by high performance liquid chromatography(HPLC).Methods Analysis was performed on a Waters Symmetry C18(250 mm×4.6 mm,5μm).The mobile phase was considered of 0.1%phosphoric acid solution and acetonitrile(75:25),with a flow rate of 1.0 mL/min and wavelength of 240 nm.It was quantified by external standard method.Results Two substances were separated effectively,sulfaquinoxaline and diaveridine had good linearity over the range of 5.0-100μg/mL and 1.0-20μg/mL,respectively(r^2=0.99998).Sulfaquinoxaline was added at 0.2 mg/mg,with an average recovery of 97.86%(RSD=0.8%).Dimethoxybenzidine was added at 0.04 mg/mg,with an average recovery of 95.14%(RSD=1.9%).Conclusion This method is accurate,rapid and repeatable,which can be used for the simultaneous determination of sulfaquinoxaline and diaveridine in sulfaquinoxaline and diaveridine premix.

关 键 词：磺胺喹噁啉 二甲氧苄啶 高效液相色谱法 

分 类 号：O657.72[理学—分析化学] S859.79[理学—化学]