光谱法研究呋喃妥因与牛血清白蛋白的相互作用  

Interaction between Nitrofurantoin and Bovine Serum Albumin by Spectroscopy

作　　者：赵刚[1] 胡雪健 何茜 黄曦瑶 ZHAO Gang;HU Xue-jian;HE Qian;HUANG Xi-yao(College of Chemistry and Chemical Engineering,Bohai University,Jinzhou 121013,China)

机构地区：[1]渤海大学化学化工学院,辽宁锦州121013

出　　处：《化学试剂》2020年第6期677-680,共4页Chemical Reagents

基　　金：国家自然科学基金资助项目(41602351)。

摘　　要：呋喃妥因(NFT)属于硝基呋喃类抗生素,由于其具有遗传毒性和致癌性,已被许多国家列为禁药。采用荧光光谱法、紫外-可见光谱法、同步荧光光谱法、时间分辨荧光光谱法等研究了NFT和牛血清白蛋白(BSA)之间相互作用机制。实验结果表明:NFT对BSA的荧光猝灭主要为静态猝灭;25℃时二者的结合常数与结合位点数分别为6.02×10^4 L/mol和1.28;热力学参数焓变(ΔH)与熵变(ΔS)分别为-101.3 kJ/mol和-0.28 J/(mol·K),说明NFT与BSA之间的作用力主要为范德华力和氢键;位点竞争实验结果表明NFT结合在BSA亚结构域ⅡA的位点Ⅰ。紫外光谱法和同步荧光光谱法实验结果表明,NFT诱导BSA的构象发生变化。Nitrofurantoin(NFT)is a nitrofuran antibiotic.It has been banned in many countries due to its genetic toxicity and carcinogenicity.The interaction mechanism between NFT and bovine serum albumin(BSA)was studied by fluorescence spectroscopy,UV-Vis spectroscopy,synchronous fluorescence spectroscopy,time resolved fluorescence spectroscopy.The results showed that the BSA fluorescence quenching was mainly static quenching by NFT.The binding constant and the number of binding sites were 6.02×10^4 L/mol and 1.28 at 25℃.The enthalpy change(ΔH)and entropy(ΔS)were-101.3 kJ/mol and-0.28 J/(mol·K),which indicated that the forces were van der Waals forces and hydrogen bonds between NFT and BSA.The results of site competition experiments showed that NFT binds to BSA siteⅠ(subdomainⅡA).The results of UV-Vis spectroscopy and synchronous fluorescence spectroscopy showed that the BSA conformation changed induced by NFT.

关 键 词：呋喃妥因 牛血清白蛋白 荧光光谱法 紫外-可见光谱法 相互作用 

分 类 号：O657.3[理学—分析化学]