肝细胞癌癌组织Hic-5和COL1A1表达及其对术后生存的预测价值  

Poor prognosis of patients withhepatocellular carcinoma after hepatectomy with high cancerous tissue Hic-5 and COL1A1 expression

作　　者：顾世玉[1] 秦文举 高建芝[2] 薛明辉[3] Gu Shiyu;Qin Wenju;Gao Jianzhi(Department of Gastroenterology,Third Affiliated Hospital,Xinxiang Medical College,Xinxiang 453000,Henan Province,China)

机构地区：[1]新乡医学院第三附属医院消化内科,河南省新乡市453000 [2]解放军总医院病理科 [3]新乡医学院第一附属医院普外科,河南省新乡市453000

出　　处：《实用肝脏病杂志》2020年第4期576-580,共5页Journal of Practical Hepatology

基　　金：河南省自然科学基金资助项目(2012CJ021)。

摘　　要：目的探讨肝细胞癌(HCC)组织氧化氢诱导的克隆基因-5(Hic-5)和Ⅰ型胶原α1链(COL1A1)表达及其对HCC患者术后生存时间的预测价值。方法2013年9月~2016年7月我院行肝癌切除术的HCC患者97例,术后取癌组织和癌旁肝组织。采用RT-PCR法和免疫组化法分别检测组织Hic-5和COL1A1 mRNA和蛋白表达。随访3年。结果HCC患者癌组织Hic-5蛋白表达阳性率为53.6%,显著高于癌旁组织(16.5%,P<0.05),其mRNA阳性率为66.0%,也显著高于癌旁组织(25.8%,P<0.05);HCC患者癌组织COL1A1蛋白表达阳性率为71.1%,显著高于癌旁组织(25.8%,P<0.05),其mRNA阳性率为78.4%,也显著高于癌旁组织(30.9%,P<0.05);TNMⅠ~Ⅱ期患者癌组织Hic-5 mRNA阳性率为56.4%,显著低于Ⅲ~Ⅳ期者(78.6%,P<0.05),COL1A1 mRNA阳性率为70.9%,也显著低于Ⅲ~Ⅳ期者(88.1%,P<0.05),存在门静脉癌栓患者癌组织Hic-5 mRNA阳性率为82.5%,显著高于无门静脉癌栓者(54.4%,P<0.05),COL1A1 mRNA阳性率为90.0%,显著高于无门静脉癌栓者(70.2%,P<0.05);随访的87例患者生存时间为8.2~36.0个月,中位生存时间为36.0(14.6,36.0)个月;癌组织Hic-5阳性表达患者3 a生存时间为26.3(16.1,36.0)月,显著短于Hic-5阴性者【36.0(30.6,36.0),P<0.05】,3 a无事件生存时间为21.4(11.7,36.0)月,也显著短于Hic-5阴性者【36.0(24.2,36.0),P<0.05】。结论HCC患者癌组织Hic-5阳性表达预示术后生存时间缩短,其原因可能与肿瘤分期差或门静脉存在癌栓有关。Objective The aim of this study was to analyze the expression of hydrogen peroxide inducible clone-5(Hic-5)and collagen type Ⅰ alpha gene(COL1A1)in cancerous tissues and their predictive value for postoperative survival time of patients with hepatocellular carcinoma(HCC).Methods 97 patients with HCC underwent hepatectomy in our hospital during the period between September 2013 and July 2016,and all patients were followed-up for 36 months.The RT-PCR and immunohistochemistry were performed to detect Hic-5 and COL1A1 mRNA and their proteins in cancerous and non-cancerous tissues.Results The positive rate of Hic-5 protein in cancerous tissues was significantly higher than that in adjacent liver tissues(53.6%vs.16.5,P<0.05),and the positive rate of Hic-5 mRNA was significantly higher than that in adjacent tissues(66.0%vs.25.8%,P<0.05);the positive rate of COL1A1 protein in cancerous tissues was significantly higher than that in adjacent tissues(71.1%vs.25.8%,P<0.05),and the positive rate of COL1A1 mRNA was significantly higher than that in adjacent tissues(78.4%vs.30.9%,P<0.05);the positive rate of Hic-5 mRNA in patients with TNM stage Ⅰ/Ⅱ was significantly lower than that in with stage Ⅲ/Ⅳ(56.4%vs.78.6%,P<0.05),and the positive rate of COL1A1 mRNA was significantly lower than that in with stage Ⅲ/Ⅳ(70.9%vs.88.1%,P<0.05);the positive rate of Hic-5 in patients with portal vein thrombosis was significantly higher than that in without(82.5%vs.54.4%,P<0.05),and the positive rate of COL1A1 mRNA was significantly higher than that in without(90.9%vs.70.2%,P<0.05);the medium survival in 87 followed-up patients was 8.2 to 36.0 months36.0(14.6,36.0)m;the 3-year cumulative survival in patients with positive Hic-5 expression was significantly shorter than that in with negative[26.3(16.1,36.0)m vs.36.0(30.6,36.0)m,P<0.05],and the 3-year cumulative event-free survival was also significantly shorter than that in with negative[21.4(11.7,36.0)m vs.36.0(24.2,36.0)m,P<0.05].Conclusion The positive Hic-5 expression in cance

关 键 词：肝细胞癌 肝癌切除术 氧化氢诱导的克隆基因-5 Ⅰ型胶原α1链 生存 

分 类 号：R735.7[医药卫生—肿瘤]