肠道类器官的构建及应用  

Establishment and application of gut organoids

作　　者：孙霞[1] 詹丽杏 Sun Xia;Zhan Lixing(Key Laboratory of Nutrition,Metabolism and Food Safety,Shanghai Institute of Nutrition and Health,Shanghai Institutes for Bio⁃logical Sciences,Chinese Academy of Sciences,Shanghai 200031,China)

机构地区：[1]中国科学院营养代谢与食品安全重点实验室/上海营养与健康研究所/中国科学院上海生命科学研究所,上海200031

出　　处：《结直肠肛门外科》2020年第3期251-256,共6页Journal of Colorectal & Anal Surgery

基　　金：科技部重点研发计划专项项目(2017YFC1600100);中科院战略先导科技专项A类“个性化药物-基于疾病分子分型的普惠新药研发”项目(XDA12010102);恩施州科技计划项目。

摘　　要：肠道类器官是一种来源于肠道干细胞或多能干细胞(PSC)且具备三维结构的微器官。将肠道隐窝或单个干细胞植入基质胶中,在含有多种生长因子的培养皿中生成微型肠器官。该类器官包含各种肠道上皮细胞且能够有效地模拟肠组织。自2009年首次构建出肠道类器官,就迅速成为肠道疾病研究领域的热点。本文将围绕肠道类器官的构建及应用的最新进展进行述评。Gut organoids are three-dimensional mini-guts that are derived from gut stem cells or pluripotent stem cells(PSC).Gut single crypts or stem cells,upon embedment into matrigel,are cultured under specific growth factors into self-organizing or⁃ganotypic structures.Organoids are comprised of all maj or types of gut epithelial cells and contain varying levels of physiologic similarity to native organ systems.Since first reported in 2009,gut organoids have soon become an important topic in the field of gut diseases.In this review article,we summarize the research advances in establishment and application of gut organoids.

关 键 词：肠道干细胞 类器官 肿瘤类器官 3D培养 

分 类 号：R574[医药卫生—消化系统]