下调LncRNA SNHG3表达对人胃癌细胞MGC-803增殖、侵袭、凋亡的影响及机制  

Effects of down-regulating LncRNA SNHG3 expression on proliferation,invasion,and apoptosis of human gastric cancer cells MGC-803

作　　者：谷建斌[1] 张国欣[1] 张玉斌[1] 张景承[1] 王雪平[1] 薛菲[1] GU Jianbin;ZHANG Guoxin;ZHANG Yubin;ZHANG Jingcheng;WANG Xueping;XUE Fei(The First Hospital of Shijiazhuag City,Shijiazhuang 050011,China)

机构地区：[1]石家庄市第一医院,石家庄050011

出　　处：《山东医药》2020年第19期34-38,共5页Shandong Medical Journal

摘　　要：目的探讨下调LncRNA SNHG3表达对人胃癌MGC-803细胞增殖、侵袭和凋亡的影响及可能机制。方法培养人胃癌MGC-803细胞,随机分为空白对照组、阴性对照组、SNHG3-siRNA组。SNHG3-siRNA组、阴性对照组分别转染LncRNA SNHG3-siRNA、阴性对照序列,空白对照组不予转染。应用siRNA技术下调LncRNA SNHG3的表达,通过CCK-8法、克隆形成实验、Transwell迁移和侵袭实验、流式细胞技术探讨LncRNA SNHG3表达对人胃癌细胞MGC-803增殖、迁移、侵袭和凋亡的影响。蛋白质印迹法(Western blotting)测定下调LncRNA SNHG3表达后胃癌细胞增殖和转移相关信号传导与活化转录因子3(STAT3)、基质金属蛋白酶2(MMP-2)、c-Myc和p-STAT3蛋白表达情况。结果与正常胃黏膜上皮细胞GES1相比,人胃癌细胞株MGC-803的LncRNA SNHG3相对表达量增高(P<0.01)。siRNA转染胃癌细胞株MGC-803后SNHG3-siRNA组LncRNA SNHG3相对表达量0.27±0.03,低于空白对照组、阴性对照组(P均<0.05)。下调LncRNA SNHG3表达,CCK-8法检测结果示,SNHG3-siRNA组OD450为0.4109±0.0015,siRNA组的增殖能力低于阴性对照组、空白对照组(P均<0.01)。SNHG3-siRNA组、阴性对照组、空白对照组的克隆形成率分别为5.89%±0.44%、9.48%±1.17%、10.00%±0.76%,SNHG3-siRNA组克隆形成率低于阴性对照组、空白对照组(P均<0.01)。MGC-803细胞转染SNHG3 siRNA 24 h后,细胞G2/M期比例下降至7.15%±1.08%,与阴性对照组相比差异有统计学意义(P<0.01)。下调LncRNA SNHG3表达后,MGC-803细胞凋亡率上升至10.73%±0.77%,与空白对照组(1.54%±0.57%)、阴性对照组(2.34%±0.84%)相比升高(P均<0.001)。Transwell迁移实验结果显示,SNHG3-siRNA组、阴性对照组、空白对照组迁移到滤膜下表面的细胞数分别为(38.6±1.5)、(148.6±10.2)、(157.0±18.0)个/视野,SNHG3-siRNA组迁移细胞数低于阴性对照组、空白对照组(P均<0.01)。SNHG3-siRNA组、阴性对照组、空白对照组迁移到滤膜下表面的细胞数�Objective To investigate the effects of down-regulating the expression of LncRNA SNHG3 on the proliferation,invasion,and apoptosis of human gastric cancer MGC-803 cells and its possible mechanism.Methods MGC-803 cells were randomly divided into the blank control group,negative control group,and SNHG3-siRNA group.SNHG3-siRNA interference sequence and negative control sequence were transfected into the SNHG3-siRNA group and negative control group,respectively,but the cells in the blank control group were not treated.SiRNA technology was used to down-regulate the expression of LncRNA SNHG3,and qRT-PCR was used to verify the interference efficiency of si-SNHG3.CCK-8,clone formation,cell scratch test,Transwell migration and invasion tests,and flow cytometry were used to investigate the effects of LncRNA SNHG3 on the proliferation,invasion,and apoptosis of human gastric cancer MGC-803 cells.Western blotting was used to observe the expression of STAT3,MMP-2,c-Myc,and p-STAT3 in the gastric cancer cells.Results The relative expression of LncRNA SNHG3 in MGC-803 cells was higher than that in the gastric normal mucosal epithelial cells GES1(P<0.01).The relative expression of LncRNA SNHG3 in the SNHG3-siRNA group was 0.27±0.03,which was significantly lower than those in the blank control group and negative control group(both P<0.05).After down-regulating the expression of LncRNA SNHG3,the OD450 value of the SNHG3-siRNA group was 0.4109±0.0015 by CCK-8.The proliferation ability of the siRNA group was significantly lower than that of the negative control group and blank control group(P<0.01).The clone formation rates of the SNHG3-siRNA group,negative control group,and blank control group were 5.89%±0.44%,9.48%±1.17%,10.00%±0.76%,respectively.The clone formation rate of the SNHG3-siRNA group was significantly lower than those of the negative control group and blank control group(both P<0.01).After SNHG3 siRNA was transfected into MGC-803 cells for 24 hours,the percentage of cells in the G2/M phase decreased to 7.15%±1.08

关 键 词：胃癌 长链非编码RNA 核仁小分子RNA宿主基因3 细胞增殖 细胞侵袭 

分 类 号：R735.2[医药卫生—肿瘤]