新橙皮甙二氢查尔酮与HSA/BSA的相互作用机制研究  

Study on the Interaction Mechanism Between Neohesperidin Dihydrochalcone and Different Organisms(HSA/BSA)

作　　者：梁健丹 李海梅 罗华健 肖琦 杨晶 黄珊 LIANG Jian-dan;LI Hai-mei;LUO Hua-jian;XIAO Qi;YANG Jing;HUANG Shan(School of Chemistry and Materials Science,Nanning Normal University,Nanning 530001,China)

机构地区：[1]南宁师范大学化学与材料学院,广西南宁530001

出　　处：《南宁师范大学学报（自然科学版）》2020年第2期29-36,共8页Journal of Nanning Normal University：Natural Science Edition

基　　金：国家自然科学基金地区基金项目(21763005);国家自然科学基金地区基金项目(21463009);广西壮族自治区研究生创新计划项目(YCSW2019181)资助.

摘　　要：新橙皮甙二氢查尔酮(Neosperidin dihydrochalcone,NHDC)是查尔酮的衍生物,作为一种新型甜味剂,它口味清凉,在食品工业中有着广泛的应用。该研究利用荧光光谱法与圆二色光谱法探究了天然甜味剂新橙皮甙二氢查尔酮与HSA(人血清白蛋白)/BSA(牛血清白蛋白)的相互作用机制及其对HSA/BSA的构象影响。研究结果表明:NHDC均使HSA/BSA的内源荧光发生静态猝灭,作用力主要为氢键和范德华力,两个反应均为自发反应。NHDC对HSA/BSA的二级构象及氨基酸残基微环境有显著的影响。Neohesperidin dihydrochalcone(NHDC)is a derivative of chalcone.As a new sweetener,Neohesperidin dihydrochalcone(NHDC)is widely used in food industry because of its cool taste.The research team fluorescence spectroscopy and circular dichroism spectroscopy were used to investigate the interaction mechanism between the natural sweetener neosperidin dihydrochalcon(NHDC)and HSA/BSA and its conformational influence on HSA/BSA.The results show that:NHDC quenches the endogenous fluorescence of HSA/BSA statically.The main forces are hydrogen bonding and van der Waals force.Both reactions are spontaneous reactions.NHDC has a significant effect on the secondary conformation of HSA/BSA and the microenvironment of amino acid residues.

关 键 词：食品添加剂 NHDC 血清白蛋白 

分 类 号：O641.4[理学—物理化学] TQ461[理学—化学]