Activating PPARy Increases NQO1 and γ-GCS Expression via Nrf2 in Thrombin-activated Microglia  

作　　者：Hang HANG Li-kun WANG Si-ying REN An-jun SONG Guo-feng WU 

机构地区：[1]Department of Emergency,The Affiliated Hospital of Guizhou Medical University,Guiyang 550004,China

出　　处：《Current Medical Science》2020年第1期55-62,共8页当代医学科学（英文）

基　　金：grants from the National Natural Science Foundation of China(No.81560222);the Guizhou Science and Technology Foundation(No.[2017]7187,and No.[2013]2043).

摘　　要：The present study aimed to explore the molecular mechanisms underlying the increase of nicotinamide adenine dinucleotide phosphate:quinine oxidoreductase 1(NQO1)and y-glutamylcysteine synthetase(γ-GCS)in brain tissues after intracerebral hemorrhage(ICH).The microglial cells obtained from newborn rats were cultured and then randomly divided into the normal control group(NC group),model control group(MC group),rosiglitazone(RSG)intervention group(RSG group),retinoic-acid intervention group(RSG+RA group),and sulfbraphane group(RSG+SF group).The expression levels of NQO1,γ-GCS,and nuclear factor E2-related factor 2(Nrf2)were measured by real-time polymerase chain reaction(RT-PCR)and Western blotting,respectively.The results showed that the levels of NQO1,γ-GCS and Nrf2 were significantly increased in the MC group and the RSG group as compared with those in the NC group(P<0.01).They were found to be markedly decreased in the RSG+RA group and increased in the RSG+SF group when compared with those in the MC group or the RSG group(P<0.01).The RSG+SF group displayed the highest levels of NQO1,γ-GCS,and Nrf2 among the five groups.In conclusion,a medium dose of RSG increased the anti-oxidative ability of thrombinactivated microglia by increasing the expression of NQO1 and γ-GCS.The molecular mechanisms underlying the increase of NQO1 and γ-GCS in thrombin-activated microglia may be associated with the activation of Nrf2.

关 键 词：ROSIGLITAZONE peroxisome proliferator-activated receptor y nuclear factor E2-related factor 2 nicotinamide adenine dinucleotide phosphate:quinine oxidoreductase γ-glutamylcysteine synthetase 

分 类 号：R58[医药卫生—内分泌]