Regeneration Function Analysis of GmESR1 in Transgenic Soybean  

作　　者：Li Mo Pan Xiao-cheng Liu Yang Li Yuan-ming Li Hong-wei Liu Wei Su An-yu Wu Xiao-xia 

机构地区：[1]College of Agriculture,Northeast Agricultural University,Harbin 150030,China [2]65301 Farm and Sideline Bases of Army,Wudalianchi 164100,Heilongjiang,China [3]College of Resources and Environmental Sciences,Northeast Agricultural University,Harbin 150030,China [4]College of Heilongjiang Green Food Research Institute,Harbin 150030,China

出　　处：《Journal of Northeast Agricultural University(English Edition)》2020年第2期1-8,共8页东北农业大学学报（英文版）

基　　金：Supported by Creative Research Groups of Heilongjiang Province of China(JC2016004);the National Key R&D Program of China(2016YFD0100201);Harbin Science Technology Project(2015RQXXJ018)。

摘　　要：ENHANCER OF SHOOT REGENERATION(ESR1)is an important regulator of plant regeneration in vitro,which promotes regeneration of plant.In this study,transgenic positive plants with normal expression of proteins were screened by molecular assay.Through the study of the transgenic plants and the control Dongnong 50,the difference between immature embryo-induced callus and induced shoot bud was observed.The increase in callus weight indicated that GmESR1 gene accelerated the formations of shoot buds.By measuring the changes of hormone in the process of induction callus of transgenic plants,it was found that the contents of indole-3-acetic acid(IAA)and zeatin(ZT)in transgenic lines were significantly increased.It could be concluded that GmESR1 gene promoted the accumulation of hormone and affected regeneration process.In addition,this study also verified the interaction between GmBIM1 gene and GmESR1 gene by bimolecular fluorescence complementation(BiFC).

关 键 词：transgenic soybean GmESR1 REGENERATION indole-3-acetic acid(IAA) zeatin(ZT) 

分 类 号：S565.1[农业科学—作物学]