S100A8、S100A9蛋白在健康和实验性牙周炎组织中的表达分布初探  

Preliminary study on the expression and distribution of S100A8 and S100A9 in healthy and experimental periodontitis tissues

作　　者：郜洪宇 黄宝鑫[1,3] 侯建霞[1] 孟焕新[1] Gao Hongyu;Huang Baoxin;Hou Jianxia;Meng Huanxin(Department of Periodontology,Peking University School and Hospital of Stomatology&National Clinical Research Center for Oral Diseases&National Engineering Laboratory for Digital and Material Technology of Stomatology&Beijing Key Laboratory of Digital Stomatology,Beijing 100081,China;Department of Periodontology,Stomatological Hospital,Tianjin Medical University,Tianjin 300070,China;Department of Oral Implantology,Guanghua School of Stomatology,Hospital of Stomatology,Sun Yat-sen University&Guangdong Provincial Key Laboratory of Stomatology,Guangzhou 510055,China)

机构地区：[1]北京大学口腔医学院·口腔医院牙周科,国家口腔疾病临床医学研究中心,口腔数字化医疗技术和材料国家工程实验室,口腔数字医学北京市重点实验室,100081 [2]天津医科大学口腔医院牙周科,300070 [3]中山大学光华口腔医学院·附属口腔医院种植科·广东省口腔医学重点实验室,广州510055

出　　处：《中华口腔医学杂志》2020年第6期402-407,共6页Chinese Journal of Stomatology

基　　金：天津市教委科研计划(2016YD16)。

摘　　要：目的观察S100A8、S100A9蛋白在比格犬健康及实验性牙周炎组织中的表达分布特点。方法将6只比格犬的一侧下颌第二磨牙通过拴线法诱导实验性牙周炎模型(结扎组),另一侧下颌第二磨牙保持口腔卫生(健康对照组),应用免疫组化法检测S100A8、S100A9在6只比格犬健康及实验性牙周炎组织中的表达;免疫细胞化学法检测两种蛋白亚基在人牙龈成纤维细胞(human gingival fibroblasts,hGF)(来自3例牙冠延长术切除的牙龈组织)、人牙周膜细胞(human periodontal ligament cells,hPDLC)(来自3例因正畸拔除的前磨牙或第三磨牙的牙周膜细胞)中的表达。结果实验性牙周炎诱导第12周,结扎组探诊深度[(3.86±0.14)mm]显著高于健康对照组[(2.11±0.28)mm,P<0.01];比格犬健康牙周组织中S100A8、S100A9主要表达于牙龈上皮细胞、中性粒细胞,且在结合上皮处呈强阳性表达;实验性牙周炎组织中除牙龈上皮、中性粒细胞外,两种蛋白还诱导表达于牙龈成纤维细胞、牙周膜细胞、微血管内皮细胞及骨髓成纤维细胞;hGF及hPDLC中均可检测到S100A8、S100A9的表达。结论实验性牙周炎使S100A8、S100A9的表达范围更大,表达S100A8、S100A9的细胞类型更多。Objective To investigate the systemic expression profile of S100A8 and S100A9 in healthy and inflamed periodontal tissues.Methods Experimental periodontitis models were established by ligations around the mandibular second molars of six beagle dogs for 12 weeks(ligation group).The mandibular second molars on the opposite side were kept clean(healthy control group).The expressions of S100A8 and S100A9 in healthy and inflamed periodontal tissues of six beagle dogs were examined by immunohistochemistry.The expressions of S100A8 and S100A9 in primary human gingival fibroblasts(hGF)from 3 subjects and human periodontal ligament cells(hPDLC)from 3 other subjects were detected by immunocytochemistry.Results After the ligation for 12 weeks,the mean probing depth of ligation group[(3.86±0.14)mm]was significantly higher than that of healthy control group[(2.11±0.28)mm](P<0.01).Results of immunohistochemistry analysis indicated that S100A8 and S100A9 could be expressed in gingival epithelial cells and might infiltrated neutrophils in the healthy periodontium.Except for the gingival epithelial cells and neutrophils,both proteins were induced and expressed in gingival fibroblasts,periodontal ligament cells,microvascular endothelial cells and bone marrow fibroblasts under inflammatory conditions.The distribution of S100A8 and S100A9 differed in the healthy oral gingival epithelium(OGE),which becomes consistent in inflamed OGE.Additionally,the expressions of S100A8 and S100A9 were confirmed in primary hGF and hPDLC.Conclusions Periodontal inflammation might enlarge the expression scope of S100A8 and S100A9 and enrich multiple cells with expressions of S100A8 and S100A9.

关 键 词：牙周炎 S100A8 S100A9 牙龈成纤维细胞 牙周膜细胞 

分 类 号：R78[医药卫生—口腔医学]