胡颓子叶中槲皮素和山柰酚薄层鉴别及其总黄酮、免疫活性研究  

TLC Indentification of Quercetin and Kaempferol and Determination of Total Flavonoids,Invitro Immunostimulatory Activities Analysis of Elaeagnus pungens

作　　者：周美 王道平 杨莹 颜秋晓 安巧 胡华林 杨艳[1] ZHOU Mei;WANG Daoping;YANG Ying;YAN Qiuxiao;AN Qiao;HU Hualin;YANG Yan(State Key Laboratory of Functions and Applications of Medicinal Plants/The Key Laboratory of Chemistry for Natural Produets of Guizhou Provineeand Chinese Aeademy of Seienees,Guizhou Medcial University,Guiyang 550014,China;Guizhou Bailing Enterprise Group Pharmaceutical CO.Ltd.,Anshun 561000,China)

机构地区：[1]贵州医科大学省部共建药用植物功效与利用国家重点实验室/贵州省中国科学院天然产物化学重点实验室,贵州贵阳550014 [2]贵州百灵企业集团制药股份有限公司,贵州安顺561000

出　　处：《信阳师范学院学报（自然科学版）》2020年第3期410-415,共6页Journal of Xinyang Normal University(Natural Science Edition)

基　　金：贵州省科技计划项目(黔科合LH字[2016]7411号);贵州省科技计划项目(黔科合支撑[2019]2753号);贵州省科技厅基础研究计划项目(黔科合基础[2019]1013);贵州省科技成果转化项目(黔科合成转字[2015]5206号);贵州省科技计划项目(黔科合支撑[2018]2352)。

摘　　要：采用薄层色谱法对胡颓子叶中槲皮素和山柰酚进行定性鉴别,采用紫外-可见分光光度法测定总黄酮,并通过小鼠巨噬细胞(RAW264.7)模型评价其体外免疫活性.结果表明:在薄层色谱中能检测到槲皮素和山柰酚;总黄酮在0.008~0.054 g/L范围内具有良好的线性关系(R=0.9991),回收率为99.6%,RSD为1.32%;胡颓子叶总黄酮能有效促进免疫细胞增殖,可显著抑制LPS诱导的巨噬细胞RAW 264.7中TNF-α的分泌量,表明其具有较好的免疫活性.Quercetin and kaempferol were identified by TLC.The content of total flavonoids was detected by UV.The invitro immunostimulatory activities of Elaeagnus pungens were investigated by using the immune activity analysis cell model(RAW264.7 macrophages).The results showed that:quercetin and kaempferol could be detected by TLC;the calibration curve showed good linearity over the range of 0.008~0.054 g/L(R=0.9991);the average recovery was 99.6%with RSD of 1.32%;the research of immunological activity of the general flavone of Elaeagnus pungens showed that it could effectively promote the proliferation of immune cells,and significantly inhibit the secretion of TNF-αin LPS-induced macrophages RAW 264.7,indicating its remarkable immunological activity.

关 键 词：胡颓子 槲皮素 山柰酚 薄层鉴别 总黄酮 免疫活性 

分 类 号：TS201.1[轻工技术与工程—食品科学]