RP-HPLC法同时测定小儿柴桂退热口服液中7个成分的含量  

Simultaneous determination of seven constituents in Xiao’er Chaigui Tuire oral liquid by RP-HPLC

作　　者：王辉 牟亦 徐宁 姚文涛 王丽 吴佳瑛 WANG Hui;MOU Yi;XU Ning;YAO Wen-tao;WANG Li;WU Jia-ying(People's Hospital of Anji,Huzhou 313310,China;Traditional Chinese Medicine Hospital of Ningbo,Ningbo 315010,China)

机构地区：[1]安吉县人民医院,湖州313310 [2]宁波市中医院,宁波315010

出　　处：《药物分析杂志》2020年第5期814-820,共7页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立同时测定小儿柴桂退热口服液中没食子酸、葛根素、芍药苷、黄芩苷、柴胡皂苷a、柴胡皂苷d和桂皮醛的含量测定方法。方法:采用反相高效液相色谱法,色谱柱为Thermo ODS C18(250 mm×4.6mm,5μm),以乙腈-0.1%磷酸水溶液为流动相,梯度洗脱,流速1.0 mL·min^-1,检测波长254 nm(没食子酸、葛根素、芍药苷)、278 nm(黄芩苷)、210 nm(柴胡皂苷a、柴胡皂苷d)、290 nm(桂皮醛),柱温25℃,进样量20μL。结果:没食子酸、葛根素、芍药苷、黄芩苷、柴胡皂苷a、柴胡皂苷d和桂皮醛检测质量浓度的线性范围分别为0.0078~0.0933、0.1544~1.8532、0.0313~0.3756、0.0704~0.8450、0.0434~0.5212、0.0232~0.2789、0.0131~0.1577 mg·mL^-1(r为0.9994~0.9999);平均加样回收率分别为101.1%、100.3%、98.8%、101.2%、98.3%、99.9%、99.4%(RSD<2.0%,n=6)。5批小儿柴桂退热口服液样品中测得的上述7个成分平均含量分别为0.200~0.221、4.146~4.200、0.818~0.856、1.698~1.754、1.086~1.236、0.586~0.732、0.084~0.320 mg·mL^-1。结论:该方法操作简便,结果准确,重复性好,可用于小儿柴桂退热口服液的质量控制。Objective:To develop a method for simultaneous determination of gallic acid,puerarin,paeoniflorin,baicali,saikosaponin a,saikosaponin d and cinnamaldehyde in Xiao’er Chaigui Tuire oral liquid by RPHPLC.Methods:The analysis was carried out on a Thermo ODS C18(250 mm×4.6 mm,5μm)column with the mobile phase consisting of acetonitrile and 0.1%phosphoric acid in a gradient mode at a flow rate of 1.0 mL·min^-1.The detection wavelengths were set at 254 nm for gallic acid,puerarin and paeoniflorin,278 nm for baicali,210 nm for saikosaponin a,saikosaponin d and 290 nm for cinnamaldehyde.The column temperature was set at 25℃and the injection volume was 20μL.Results:The linear rangs were 0.0078-0.0933,0.1544-1.8532,0.0313-0.3756,0.0704-0.8450,0.0434-0.5212,0.0232-0.2789,0.0131-0.1577 mg·mL^-1 for gallic acid,puerarin,paeoniflorin,baicali,saikosaponin a,saikosaponin d and cinnamaldehyde,respectively(r=0.9994-0.9999).Average recoveries were 101.1%,100.3%,98.8%,101.2%,98.3%,99.9%and 99.4%,respectively(RSD<2.0%,n=6).The average contents of the above 7 components in 5 samples were 0.200-0.221,4.146-4.200,0.818-0.856,1.698-1.754,1.086-1.236,0.586-0.732 and 0.084-0.320 mg·mL^-1,respectively.Conclusions:The established method is simple,accurate and reproducible.It can be used for quality control of Xiao’er Chaigui Tuire oral liquid.

关 键 词：小儿柴桂退热口服液 没食子酸 葛根素 芍药苷 黄芩苷 柴胡皂苷 桂皮醛 反相高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]