胃肠道间质瘤组织中Gli-1、p-AKT、p-ERK的表达及意义  

The expression of Gli-1,p-AKT and p-ERK in GIST tissues and its indications

作　　者：祁义军[1] 赵文娣[2] 王正林[1] 杨书瀚 汪正广[1] QI Yi-jun;ZHAO Wen-di;WANG Zheng-lin(The First Affiliated Hospital of Anhui Medical University generalsurgery,Hefei 230022,Anhui;不详)

机构地区：[1]安徽医科大学第一附属医院普外科,安徽合肥230022 [2]安徽医科大学病理学教研室,安徽合肥230022

出　　处：《安徽卫生职业技术学院学报》2020年第3期29-31,共3页Journal of Anhui Health Vocational & Technical College

基　　金：安徽高校自然科学研究项目(编号:KJ2019A0250)。

摘　　要：目的:分析Shh、PI3K和MAPK信号通路中的关键因子Gli-1、p-AKT和p-ERK在胃肠道间质瘤(GIST)组织中的表达情况,以探讨上述三条信号通路表达之间的相关性。方法:收集某医院手术切除、资料完整的GIST石蜡组织标本145例,Western blot检测GIST组织中Gli-1、p-AKT及p-ERK的表达水平,分析上述三种蛋白的表达及其相关性。结果:Gli-1、p-AKT和p-ERK在不同危险度分级的GIST中均有表达,且其表达水平与肿瘤的危险度分级密切相关,表达具有一致性。结论:Shh、PI3K和MAPK信号通路的关键因子Gli-1、p-AKT和p-ERK广泛表达于GIST中,上述信号通路参与了GIST的发生发展过程。Shh信号通路在GIST中所起作用可能是通过与PI3K通路和MAPK通路之间的交叉调控机制实现的。Objective:To examine the expression levels of key factors in the Shh,PI3K/AKT and MAPK signaling pathways,Gli-1,p-AKT and p-ERK in GIST tissues,and to explore the correlation between the expression of these three signaling pathways.Methods:145 formalin-fixed and paraffin-embedded GIST tumor tissues from patients that underwent surgery treatment at the hospital and with complete records were collected.The expression levels of Gli-1,p-AKT and p-ERK in GIST tissues were assayed with western blot,and the correlation between these three protein expression levels were analyzed.Results:Gli-1,p-AKT and p-ERK were expressed in GIST of different risk classifications,and their expression levels were highly correlated and consistent.Conclusion:The key factors in the Shh,PI3K/AKT and MAPK signaling pathways,Gli-1,p-AKT and p-ERK were widely expressed in GIST,and these signaling pathways participated in the tumorigenesis and development of GIST.The Shh pathway may exert its effect on GIST through the mechanism of crosstalk regulation between the PI3K and MAPK pathways.

关 键 词：胃肠道间质瘤 SHH信号通路 PI3K/AKT信号通路 MAPK信号通路 

分 类 号：R57[医药卫生—消化系统]