考马斯亮蓝G250法测定蛋白质含量的教学实践及方法学探讨  

Teaching Practice and Methodological Investigation of Protein Content Determination Using Coomassie Brilliant Blue G250

作　　者：祝连彩[1] 唐士金 周丽[1] ZHU Lian-cai;TANG Shi-jin;ZHOU Li(Modern Experiment Teaching Center,Bioengineering College,Chongqing University,Chongqing 400030,China)

机构地区：[1]重庆大学生物工程学院现代实验教学中心,重庆400030

出　　处：《教育教学论坛》2020年第23期266-269,共4页Education And Teaching Forum

摘　　要：该文研究之目的:考马斯亮蓝G250法测定蛋白质含量是生物化学实验教学的基本实验之一。为了更好地开展实验教学,对实际教学中发现的该方法组间稳定性及线性相关性存在的问题进行研究和探讨。之方法:在对问题出现原因进行解析的基础上,对影响实验稳定性的因素和线性范围进行了实验研究。之结果:发现该方法的显色稳定时间为60min,线性范围为1.0-30.0μg/mL。之结论:将研究成果引入实验教学,将有利于学生对于实验原理的理解,并消除学生对组间实验结果差异大的疑虑,提高实验教学效果。The Coomassie Brilliant Blue Method is widely used for the determination of protein and is also one of the basic experiments of biochemistry experiment teaching.In order to carry out the experimental teaching effectively,we studied the factors affecting the stability of the experiment and the linear range.The results show that the chromogenic reaction reaches to stability after 60 min and the linear range of this method is 1.0-30.0μg/mL.The results of the study will be beneficial to understanding the experimental principle,eliminating the bewilderment of students on the large difference among difference groups,and improving the effect of experiment teaching.

关 键 词：蛋白质含量 考马斯亮蓝G250 显色稳定性 Lambert—Beer定律 线性范围 

分 类 号：G642[文化科学—高等教育学]