大鼠鞘内给药方法比较及改进  

Comparison and Improvement of Intrathecal Administration Methods in Rats

作　　者：崔秀玉 周岩 CUI Xiuyu;ZHOU Yan(Center of Parkinson's Disease,Beijing Institute for Brain Disorders,Center for Brain Disorders Research,Capital Medical University.,Beijing 100069,China;Department of Neurobiology,Basic medicine Institute,Capital Medical University,Beijing 100069,China)

机构地区：[1]北京脑重大疾病研究院帕金森病研究所,首都医科大学脑重大疾病研究中心,北京100069 [2]首都医科大学基础医学院神经生物学系,北京100069

出　　处：《实验动物科学》2020年第2期31-36,共6页Laboratory Animal Science

基　　金：北京市教育委员会科技计划面上项目(No.KM201610025014)。

摘　　要：目的通过实验对比研究,介绍一种损伤少、稳定可靠、可持续性给药的鞘内给药方法。方法大鼠72只随机分为6组:4组手术处理组(经枕寰膜置管组、经枕寰膜置管+微型渗透泵组、经腰椎椎间孔置管组、经腰椎椎间孔置管+微型渗透泵组)和2组假手术对照组(经枕寰膜置管假手术组、经腰椎椎间孔置管假手术组)。置管组导管游离端固定于皮肤。观察术后大鼠体质量、致瘫、致死、脱管等一般状态,同时检测手术前后转棒持续时间、机械刺激缩足反射阈值及热刺激缩足反射潜伏期行为学变化。结果经枕寰膜置管大鼠近50%致死、致瘫、一般状态不良(体质量严重下降、口鼻炎性分泌物)。经枕寰膜置管+微型渗透泵组大鼠情况类似。经腰椎椎间孔置管组大鼠未见致死、致瘫及体质量下降,但1/3发生脱管。腰椎椎间孔置管+微型渗透泵组大鼠未见致死、致瘫及体质量下降,全部未脱管。经枕寰膜置管可致大鼠转棒运动能力显著低于术前和对照组,其热刺激缩足反射潜伏期和机械刺激缩足反射阈值均显著低于术前和对照组。经腰椎椎间孔置管对大鼠转棒运动能力和基础痛阈均无明显影响,与对照组和术前自身比较差异均无显著性。结论经腰椎椎间孔置管结合微型渗透泵方法是鞘内给药损伤少、稳定可靠的最佳方法。Objective Based on experiments,an intrathecal administration method which is stable,reliable,easy,continuous and less damage was presented.Method Rats were randomly underwent catheter implantation either through atlanto-occipital membrane or foramen intervertebrale,the outer end of the catheters were either linked to a Alzet Osmotic Pump or just fixed to skin.According to the different treatment pattern,the animals were divided into 6 groups:4 operation groups(atlanto-occipital catheterization group,atlanto-occipital catheterization with Osmotic Pump group,foramen intervertebrale catheterization group,foramen intervertebrale catheterization with Osmotic Pump group)and 2 sham groups(atlanto-occipital catheterization sham group,foramen intervertebrale catheterization sham group).Death,paralysis,and sighs of developing motor dysfunction and ill health were abserved after surgery.Rotarod motor function,paw withdrawal threshold(PWT)and paw withdrawal latency(PWL)were also tested before and after surgery.Result Only half of the rats underwent atlanto-occipital catheterization could be used for further study,the others developed motor dysfunction,ill health(seepage of eyes and nose,weight loss)or were dead.Because of dislodging of the catheter,one-thirds of the animals underwent foramen intervertebrale catheterization were excluded from further study,the other two-thirds of the rats were qualified.While all of the rats underwent foramen intervertebrale catheterization connecting to Osmotic Pump could be used for further study.Atlanto-occipital catheterization could result in hyperalgesia and decrease of rotarod motor function.The duration of running times on rotarod,PWT and PWL were all significantly lower than that of sham group and before-operation state.Foramen intervertebrale catheterization had no significant effect on PWT,PWL and the running times on rotarod.Conclusion Iintervertebrale catheterization combining with Osmotic Pump was a stable,reliable intrathecal adminidtration method with less damage.

关 键 词：鞘内给药 大鼠 枕寰膜 腰椎椎间孔 

分 类 号：R-331[医药卫生]