石墨烯-二氧化锰-离子液体修饰玻碳电极同时测定尿液中尿酸、抗坏血酸、黄嘌呤  

Graphene/manganese dioxide/ionic liquid modified glassy carbon electrode for simultaneous determination of uric acid,ascorbic acid and xanthine in urine

作　　者：朱峰 王静 刘林海 陈宗保 ZHU Feng;WANG Jing;LIU Linghai;CHEN Zongbao(College of Chemistry and Environmental Science,Shangrao Normal University,Shangrao 334001)

机构地区：[1]上饶师范学院化学与环境科学学院,上饶334001

出　　处：《分析试验室》2020年第6期640-644,共5页Chinese Journal of Analysis Laboratory

基　　金：国家自然基金(21465020,21765018);江西省重点研发计划项目(20161BBF60042)资助。

摘　　要：采用滴涂法将石墨烯-二氧化锰分散液于玻碳电极上,然后利用循环伏安法将1-辛基-3-甲基咪唑六氟磷酸盐修饰,制得石墨烯-二氧化锰-离子液体修饰电极。研究了修饰电极对尿酸、抗坏血酸和黄嘌呤混合物电化学行为,实验结果表明,在pH 5. 0的0. 2 mol/L的磷酸缓冲溶液中,尿酸、抗坏血酸和黄嘌呤在修饰电极上有较好的电化学行为,3种分析物浓度分别在0. 5~45μmol/L,2. 5~25μmol/L,1. 0~100μmol/L范围内呈良好的线性关系,检出限分别为0. 031,1. 1,0. 21μmol/L。方法用于人体尿液中3种分析物同时检测,其RSDs均小于4. 7%,回收率分别为93. 6%~95. 3%,73. 0%~82. 0%,80. 0%~81. 8%。The glassy carbon electrode was modified by dropping graphene/manganese dioxide dispersion,electro-polymerized by 1-octyl-3-methylimidazole hexafluorophosphate to prepare the G R/MnO 2/OMIMPF6/GCEmodified electrode.The electrochemical behaviors of uric acid(UA),ascorbic acid(AA)and xanthine(XA)were studied by the modified electrode.The experimental results showed that UA,AA and XA showing strong electrochemical behavior in 0.2 mol/L phosphoric acid buffer(pH 5.0).The three analytes had good linearrelationships in the ranges of 0.5-45μmol/L,2.5-225μmol/L,1.0-100μmol/L,and the detection limits were 0.031,1.1,0.21μmol/L,respectively.The proposed method was used for the simultaneous determination of three analytes in human urine with the relative standard deviations(RSDs)of less than 4.7%and the recoveries for UA,AA,XA in the ranges of 93.6%-95.3%,73.0%-82.0%,80.0%-81.8%,respectively.

关 键 词：石墨烯-二氧化锰 1-辛基-3-甲基咪唑六氟磷酸盐 尿酸 抗坏血酸 黄嘌呤 

分 类 号：O657.1[理学—分析化学]