外源褪黑素对O3胁迫下葡萄叶片光合碳同化和光呼吸的影响  

Effects of exogenous melatonin on photosynthetic carbon assimilation and photorespiration in grapevine leaves under O3 stress

作　　者：王亚芳 陈钲文 杨兴旺 翟衡[1] 杜远鹏[1] WANG Yafang;CHEN Zhengwen;YANG Xingwang;ZHAI Heng;DU Yuanpeng(State Key Laboratory of Crop Biology,College of Horticulture Science and Engineering,Shandong Agricultural University,Taian,Shandong 271018,China)

机构地区：[1]山东农业大学园艺科学与工程学院,作物生物学国家重点实验室,山东泰安271018

出　　处：《植物生理学报》2020年第4期847-855,共9页Plant Physiology Journal

基　　金：国家自然科学基金(31572084);国家葡萄产业技术体系建设专项(CARS-29);长江学者和创新团队发展计划项目(IRT15R42)。

摘　　要：为了探讨外源褪黑素(melatonin, MT)对臭氧(O3)胁迫下葡萄(Vitisvinifera)叶片碳同化及光呼吸的影响,以一年生‘赤霞珠’葡萄盆栽苗为试材,在(110±10) nL·L^-1的O3胁迫下,研究了MT对葡萄叶片叶绿素、核酮糖-1,5-二磷酸羧化酶/加氧酶(Rubisco)活性、光呼吸速率及核酮糖-1,5-二磷酸(RuBP)加氧与羧化效率、可溶性糖和淀粉含量和蔗糖合成与代谢相关酶的影响。结果表明, MT处理显著提高了O3胁迫下叶片叶绿素含量、Rubisco活性、光呼吸速率、用于碳同化的激发电子[Je(PCR)]和用于光呼吸的激发电子[Je(PCO)]生成速率,显著提高了O3胁迫下叶片淀粉含量和可溶性酸性转化酶活性,降低了还原糖、蔗糖、总糖含量和蔗糖合成酶(合成方向)(SS-Ⅱ)、蔗糖磷酸合成酶(SPS)活性。说明外源MT显著提高O3胁迫下葡萄叶片光呼吸来耗散过剩光能,进而提高O3胁迫下葡萄叶片光合碳同化能力,提高了叶绿素含量和光合能力,进而促进光合产物淀粉的积累。In order to study the effects of exogenous melatonin on carbon assimilation and photorespiration in grape leaves under ozone(O3) stress, one-year old potted ‘Cabernet Sauvignon’ grapes were used as materials to study the effect of melatonin on grape leaf chlorophyll, ribulose-1,5-bisphosphate carboxylase/oxygenase(Rubisco) activity, photorespiration rate, ribulose-1,5-bisphosphate(RuBP) with oxygen and carboxylation efficiency, soluble sugar, starch content and sucrose metabolism related enzymes under(110±10) nL·L^-1 O3 stress. The results showed that melatonin treatment significantly increased chlorophyll content, Rubisco activity, photorespirationrate, electron flux for photosynthetic carbon reduction [Je(PCR)] and electron flux for photorespiratory carbon oxidation [Je(PCO)] in leaves under ozone stress. The content of starch and soluble acid invertase in leaves under ozone stress were significantly increased, while the content of reducing sugar, sucrose, total sugar, sucrose synthase(synthesis direction)(SS-Ⅱ) and sucrose phosphate synthase(SPS) were decreased. These results indicated that exogenous melatonin significantly improve the photorespiration of grape leaves under ozone stress to dissipate the excess light energy, so as to improve the photosynthetic carbon assimilation capacity, increase chlorophyll content and photosynthetic capacity, and induced the accumulation of photosynthetic product starch.

关 键 词：褪黑素 臭氧胁迫 葡萄叶片 光合碳同化 光呼吸 

分 类 号：S663.1[农业科学—果树学]