长链非编码RNA AWPPH调控微RNA-383-5p对子宫内膜癌细胞增殖、侵袭和迁移的影响及作用机制研究  

Effect and mechanism of LncRNA AWPPH on proliferation,invasion and migration of endometrial carcinoma cells by regulating miR-383-5p

作　　者：朱丽娟[1] 王爱华[1] 段晓艳 Zhu Lijuan;Wang Aihua;Duan Xiaoyan(First Department of Gynecology,First People’s Hospital,Shangqiu,Henan 100191,China)

机构地区：[1]河南省商丘市第一人民医院妇一科,100191

出　　处：《中华生物医学工程杂志》2019年第6期667-672,共6页Chinese Journal of Biomedical Engineering

摘　　要：目的探讨长链非编码RNA(LncRNA)AWPPH通过调控微RNA(miR)-383-5p对子宫内膜癌细胞增殖、侵袭和迁移的影响及作用机制。方法qRT-PCR检测子宫内膜上皮细胞ESC和3种子宫内膜癌细胞中AWPPH和miR-383-5p的表达。双荧光素酶报告基因检测和qRT-PCR验证MALAT1与miR-204的靶向调控关系。以HEC-1A细胞为研究对象,建立沉默AWPPH的子宫内膜癌细胞株。MTT法检测细胞存活,Transwell法检测细胞迁移和侵袭能力,免疫印迹检测增殖相关蛋白Cyclin D1、侵袭迁移相关蛋白MMP2和Wnt/β-catenin信号通路相关蛋白β-catenin、Gsk-3β及Axin2的表达。结果与子宫内膜上皮细胞ESC相比,AWPPH在3种子宫内膜癌细胞中的表达显著上调,而miR-383-5p的表达显著下调。miR-383-5p是AWPPH的靶基因,AWPPH可负性调控miR-383-5p的表达。沉默AWPPH可抑制子宫内膜癌细胞增殖、侵袭和迁移,抑制Cyclin D1和MMP2蛋白的表达。抑制miR-383-5p表达可逆转沉默AWPPH对子宫内膜癌细胞增殖、侵袭和迁移的抑制作用。沉默AWPPH通过上调miR-383-5p可抑制Wnt/β-catenin信号通路的活化。结论LncRNA AWPPH通过靶向下调miR-383-5p激活Wnt/β-catenin信号通路促进子宫内膜癌细胞的增殖、侵袭和迁移。Objective To investigate the effect and mechanism of LncRNA AWPPH on the proliferation,invasion and migration of endometrial cancer cells by regulating miR-383-5p.Methods The expression of AWPPH and miR-383-5p in endometrial epithelial ESC cells and 3 types of endometrial carcinoma cells was detected by qRT-PCR.Dual luciferase reporter assay and qRT-PCR were used to verify the targeted regulatory relationshipbetweenMALAT1 and miR-204.Using HEC-1A cells as the study subject,the AWPPH-silencing endometrial cancer cell line was established.The cell survival was detected by MTT assay.The cell migration and invasion ability were detected by Transwell method.The expression of proliferation-related protein Cyclin D1,invasion and migration related proteins MMP2,as well as Wnt/β-catenin signaling pathway-related proteinsβ-catenin,Gsk-3β and Axin2,were detected by Western blot.Results Compared with endometrial epithelial ESC cells,the expression of AWPPH was significantly up-regulated,while the expression of miR-383-5p was significantly down-regulated,in 3 types of endometrial cancer cells.miR-383-5p was identified as a target gene of AWPPH,and AWPPH was shown to negatively regulate the expression of miR-383-5p.Silencing AWPPH inhibited proliferation,invasion and migration of endometrial cancer cells,and the expression of Cyclin D1 and MMP2 proteins.Inhibition of miR-383-5p reversed the inhibitory effect of AWPPH-silencing on proliferation,invasion and migration of endometrial cancer cells.Silencing AWPPH inhibited the activation of Wnt/β-catenin signaling pathway by up-regulating miR-383-5p.Conclusion LncRNA AWPPH promotes the proliferation,invasion and migration of endometrial cancer cells by targeteddown-regulation of miR-383-5p whichin turnactivatesWnt/β-catenin signaling pathway.

关 键 词：长链非编码RNA AWPPH miR-383-5p 子宫内膜癌 迁移 侵袭 WNT/Β-CATENIN信号通路 

分 类 号：R737[医药卫生—肿瘤]