CAMKK2参与前脂肪细胞对前列腺癌侵袭力影响的功能研究  

Preadipocyte promotes cell invasion of prostate cancer by CAMKK2

作　　者：隋嘉 宋汶珂[2] 张帆 李云荣 詹学建 符浩[1] Sui Jia;Song Wenke;Zhang Fan;Li Yunrong;Zhan Xuejian;Fu Hao(Department of Urology,Nanhua Affiliated Hospital,University of South China,Hengyang 421002,China;Department of Gynaecology and Obstetrics,Nanhua Affiliated Hospital,University of South China,Hengyang 421002,China)

机构地区：[1]南华大学附属南华医院泌尿外科,衡阳421002 [2]南华大学附属南华医院妇产科,衡阳421002

出　　处：《中国医师杂志》2020年第6期834-837,842,共5页Journal of Chinese Physician

基　　金：湖南省自然科学基金(2016JJ2117)。

摘　　要：目的:探讨前脂肪细胞对前列腺癌细胞侵袭力的影响及其调控机制。方法:建立前脂肪细胞及前列腺癌细胞的共培养体系,通过Transwell侵袭实验检测前脂肪细胞对前列腺癌细胞的侵袭能力影响。qRT-PCR检测前脂肪细胞及前列腺癌细胞的共培养后前列腺癌DU145和22RV1细胞中钙/钙调蛋白依赖性蛋白激酶激酶2(CAMKK2)的表达情况。并且Transwell实验检测抑制CAMKK2后前脂肪细胞对前列腺癌细胞的侵袭力的影响。结果:前脂肪细胞3T3-L1可以促进前列腺癌细胞DU145及22RV1的侵袭(P<0.01),且可以提高前列腺癌细胞中CAMKK2的表达水平(P<0.01)。抑制CAMKK2后,前脂肪细胞对前列腺癌细胞侵袭力的增强作用受到抑制。结论:前脂肪细胞可能通过上调CAMKK2的表达来促进前列腺癌细胞的侵袭力。Objective Calcium/calmodulin-dependent kinase kinase(CAMKK2)is activated in prostate cancer(PCa).The aim of our study is to identify the effects of preadipocyte and CAMKK2 on the PCa cell invasion.Methods The coculture system between PCa cells and preadipocyte cells(3T3L1)was established and then Transwell assay was used to determine the invasive effect after coculture.Quantitative real-time polymerase chain reaction(qRT-PCR)was conducted to investigate the CAMKK2 expression in the coculture system.Results The 3T3L1 cells promoted the invasion of PCa cells(DU145 and 22RV1;P<0.01).Meanwhile,the 3T3L1 cells can increased expression of CAMKK2 in PCa cells(P<0.01).CAMKK2 reversed the invasive effects of preadipocyte cells on the PCa cells.Conclusions Our study demonstrates that the preadipocyte may promote the PCa cell invasion possibly by mediating the CAMKK2 expression.

关 键 词：前列腺肿瘤 细胞系 肿瘤 钙-钙调素依赖性蛋白激酶激酶2 前脂肪细胞 细胞迁移 

分 类 号：R737.25[医药卫生—肿瘤]