Protective Effect of Procyanidin B2 on Acute Liver Injury Induced by Aflatoxin B1 in Rats  

作　　者：DENG Zhi Jie ZHAO Jing Fang HUANG Feng SUN Gui Li GAO Wei LU Li XIAO De Qiang 

机构地区：[1]Department of Clinical Nutrition,The Third Affiliated Hospital of Guangxi Medical University,Nanning 530031,Guangxi,China [2]Department of Clinical Nutrition,Guangxi International Zhuang Medicine Hospital,Nanning 530000,Guangxi,China [3]Department of Clinical Nutrition,Liuzhou General Hospital,Liuzhou 545006,Guangxi,China [4]Department of Nutrition and Food Hygiene,School of Public Health,Guangxi Medical University,Nanning 530021,Guangxi,China [5]Guangxi Colleges and Universities Key Laboratory of Prevention and Control of Highly Prevalent Diseases,Guangxi Medical University,Nanning 530021,Guangxi,China

出　　处：《Biomedical and Environmental Sciences》2020年第4期238-247,共10页生物医学与环境科学（英文版）

基　　金：financially supported by National Natural Science Foundation of China[No.31360383]。

摘　　要：Objective This study aimed to explore the protective effect of procyanidin B2(PCB2)on acute liver injury induced by aflatoxin B1(AFB1)in rats.Methods Forty Sprague Dawley rats were randomly divided into control,AFB1,AFB1+PCB2,and PCB2 groups.The latter two groups were administrated PCB2 intragastrically(30 mg/kg body weight)for 7 d,whereas the control and AFB1 groups were given the same dose of double distilled water intragastrically.On the sixth day of treatment,the AFB1 and AFB1+PCB2 groups were intraperitoneally injected with AFB1(2 mg/kg).The control and PCB2 groups were intraperitoneally administered the same dose of dimethyl sulfoxide(DMSO).On the eighth day,all rats were euthanized:serum and liver tissue were isolated for further examination.Hepatic histological features were assessed by hematoxylin and eosin-stained sections.Weight,organ coefficient(liver,spleen,and kidney),liver function(serum alanine aminotransferase,aspartate aminotransferase,alkaline phosphatase,total bilirubin,and direct bilirubin),oxidative index(catalase,glutathione,superoxide dismutase,malondialdehyde,and 8-hydroxy-2′-deoxyguanosine),inflammation factor[hepatic interleukin-6(IL-6)m RNA expression and serum IL-6],and bcl-2/bax ratio were measured.Results AFB1 significantly caused hepatic histopathological damage,abnormal liver function,oxidative stress,inflammation,and bcl-2/bax ratio reduction compared with DMSO-treated controls.Our results indicate that PCB2 treatment can partially reverse the adverse liver conditions induced by AFB1.Conclusion Our findings indicate that PCB2 exhibits a protective effect on acute liver injury induced by AFB1.

关 键 词：Procyanidin B2 Aflatoxin B1 Acute liver injury Oxidative stress INFLAMMATION 

分 类 号：R285.5[医药卫生—中药学]